Development of live-cell imaging probes for monitoring histone modifications
Development of live-cell imaging probes for monitoring histone modifications
复制标题
DOI：
10.1016/j.bmc.2012.01.018
复制
发表时间：
2012-03-15
影响因子：
3.5
通讯作者：
Yoshida, Minoru
中科院分区：
文献类型：
--
作者：
Sasaki, Kazuki;Ito, Akihiro;Yoshida, Minoru
核小体组蛋白中发生的组蛋白翻译后修饰的组合决定了表观遗传密码。组蛋白修饰如乙酰化在细胞周期和分化过程中响应于多种信号而被动态控制，但矛盾的是，它们通过细胞分裂来维持，以将组织特异性基因表达模式赋予后代。活细胞中组蛋白修饰的动力学知之甚少，因为缺乏实时监测它们的实验工具。最近，已经开发了用于组蛋白H4乙酰化的基于FRET的成像探针，其使得能够监测在细胞周期和药物治疗期间组蛋白乙酰化的变化。进一步开发这种类型的荧光探针进行其他修饰，将使活细胞中复杂的表观遗传调控可视化成为可能。(C)2012爱思唯尔有限公司保留所有权利。
The combination of histone posttranslational modifications occurring in nucleosomal histones determines the epigenetic code. Histone modifications such as acetylation are dynamically controlled in response to a variety of signals during the cell cycle and differentiation, but they are paradoxically maintained through cell division to impart tissue specific gene expression patterns to progeny. The dynamics of histone modifications in living cells are poorly understood, because of the lack of experimental tools to monitor them in a real-time fashion. Recently, FRET-based imaging probes for histone H4 acetylation have been developed, which enabled monitoring of changes in histone acetylation during the cell cycle and drug treatment. Further development of this type of fluorescent probes for other modifications will make it possible to visualize complicated epigenetic regulation in living cells. (C) 2012 Elsevier Ltd. All rights reserved.