Induction of chondrogenesis and expression of superficial zone protein (SZP)/lubricin by mesenchymal progenitors in the infrapatellar fat pad of the knee joint treated with TGF-β1 and BMP-7
Induction of chondrogenesis and expression of superficial zone protein (SZP)/lubricin by mesenchymal progenitors in the infrapatellar fat pad of the knee joint treated with TGF-β1 and BMP-7
复制标题
DOI：
10.1016/j.bbrc.2008.08.138
复制
发表时间：
2008-11-07
影响因子：
3.1
通讯作者：
Reddi, A. Hari
中科院分区：
文献类型：
--
作者：
Lee, Sang Yang;Nakagawa, Toshiyuki;Reddi, A. Hari
表浅带蛋白（SZP）是关节软骨边界润滑的关键介质。在本研究中，我们意外地发现SZP在牛膝关节髌下脂肪垫（IFP）中表达。IFP基质细胞培养基中分泌蛋白的定量分析表明TGF-β 1和BMP-7具有显著的刺激作用。实时荧光定量PCR分析显示，TGF-β 1和BMP-7上调SZP的表达。TGF-β 1和BMP-7刺激软骨分化的IFP祖细胞合成和分泌SZP。软骨诱导21天，SZP mRNA显著上调。这些发现表明，TGF-β和BMP-7刺激SZP表达可能导致受损关节内组织的功能改善，IFP祖细胞可能是通过组织工程诱导关节软骨浅表区的潜在有用来源，用于骨关节炎所致受损关节软骨的再生（C）2008 Elsevier Inc. All rights reserved.
Superficial zone protein (SZP) is a key mediator of boundary lubrication of articular Cartilage in joints. In this investigation, we made the unexpected discovery that SZP was expressed in infrapatellar fat pad (IFP) from bovine knee. Quantitative analysis of secreted proteins in the medium of the IFP stromal cells demonstrated a significant stimulation by TGF-beta 1 and BMP-7. Real-time PCR analysis revealed the SZP expression was up-regulated by TGF-beta 1 and BMP-7. Chondrogenically differentiated IFP progenitor cells were stimulated by TGF-beta 1 and BMP-7 to synthesize and secrete SZP. SZP mRNA was significantly up-regulated by chondrogenic induction for 21 days. These findings indicate that the stimulation of SZP expression by TGF-beta and BMP-7 may lead to functional improvement of damaged intraarticular tissues and that IFP progenitor cells may be a potential useful source for inducing superficial zone of articular cartilage by tissue engineering for regeneration of damaged articular cartilage due to osteoarthritis (C) 2008 Elsevier Inc. All rights reserved.