Phototransistor-based optoelectronic tweezers for dynamic cell manipulation in cell culture media
Phototransistor-based optoelectronic tweezers for dynamic cell manipulation in cell culture media
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DOI：
10.1039/b906593h
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发表时间：
2010-01-01
期刊：
影响因子：
6.1
通讯作者：
Wu, Ming C.
中科院分区：
文献类型：
--
作者：
Hsu, Hsan-yin;Ohta, Aaron T.;Wu, Ming C.
基于光诱导介电电泳的光电子镊子（OET）已被证明是一种用于微粒子和细胞平行操作的多功能工具（P. Y。Chiou，A. T. Ohta和M. C. Wu，Nature，2005，436，370 372）。(1)然而，由于非晶硅有限的光电导性，常规OET装置不能在细胞培养基或其它高电导率生理缓冲液中操作。在本文中，我们报告了一种新的光电晶体管为基础的OET（Ph-OET）。由单晶双极结型晶体管组成，Ph-OET的光电导性比非晶硅高500倍以上。使用数字光投影仪已经证明了在磷酸盐缓冲盐水（PBS）和Dulbecco改良的Eagle培养基（DMEM）中对活HeLa和Jurkat细胞的有效细胞捕获，细胞运输速度为33 μ m/sec，表明力为14.5pN。还实现了细胞的光学浓缩和可单独寻址的细胞阵列的实时控制。还证明了对两个细胞之间的分离的精确控制。我们设想使用Ph-OET进行单细胞研究的新平台。
Optoelectronic tweezers (OET), based on light-induced dielectrophoresis, has been shown as a versatile tool for parallel manipulation of micro-particles and cells (P. Y. Chiou, A. T. Ohta and M. C. Wu, Nature, 2005, 436, 370 372).(1) However, the conventional OET device cannot operate in cell culture media or other high-conductivity physiological buffers due to the limited photoconductivity of amorphous silicon. In this paper, we report a new phototransistor-based OET (Ph-OET). Consisting of single-crystalline bipolar junction transistors, the Ph-OET has more than 500x higher photoconductivity than amorphous silicon. Efficient cell trapping of live HeLa and Jurkat cells in Phosphate Buffered Saline (PBS) and Dulbecco's Modified Eagle's Medium (DMEM) has been demonstrated using a digital light projector, with a cell transport speed of 33 mu m/sec, indicating a force of 14.5 pN. Optical concentration of cells and real-time control of individually addressable cell arrays have also been realized. Precise control of separation between two cells has also been demonstrated. We envision a new platform for single cell studies using Ph-OET.