Supported membrane formation, characterization, functionalization, and patterning for application in biological science and technology.
Supported membrane formation, characterization, functionalization, and patterning for application in biological science and technology.
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DOI：
10.1002/9780470559277.ch100131
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发表时间：
2010-12-01
影响因子：
--
通讯作者：
Groves, Jay T
中科院分区：
文献类型：
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作者：
Lin, Wan-Chen;Yu, Cheng-Han;Groves, Jay T
支撑膜，作为一个单一的连续的脂质双分子层形成的固体基质，如二氧化硅，已被广泛用作蛋白质-蛋白质和细胞-细胞相互作用的模型，以研究在界面上的分子相互作用和质膜的异质性。支撑膜系统的优点包括控制膜组成的能力和它与各种表面敏感的显微镜和光谱技术的相容性。微纳米技术的最新进展极大地扩展了支撑膜的使用，以解决细胞生物学中的关键问题。虽然支持膜可以很容易地通过囊泡融合，样品需要仔细准备这个过程是有效的。在这个单元的协议全面描述程序，准备，功能化，并表征支持膜。Curr.方案2：235-269 © 2010，John Wiley & Sons，Inc.
Supported membranes, formed as a single continuous lipid bilayer on a solid substrate, such as silica, have been used extensively as a model for protein-protein and cell-cell interaction, to study the molecular interactions at interfaces and the heterogeneities of plasma membranes. The advantages of a supported membrane system include the ability to control membrane composition and the compatibility it has with various surface-sensitive microscopic and spectroscopic techniques. Recent advances in micro- and nanotechnology have greatly extended the use of supported membranes to address key questions in cell biology. Although supported membranes can be easily made by vesicle fusion, the samples need careful preparation for this process to be efficient. The protocols in this unit comprehensively describe procedures to prepare, functionalize, and characterize supported membranes. Curr. Protoc. Chem. Biol. 2:235-269 © 2010 by John Wiley & Sons, Inc.