Altered humoral immunity to mycobacterial antigens in Japanese patients affected by inflammatory demyelinating diseases of the central nervous system.
Altered humoral immunity to mycobacterial antigens in Japanese patients affected by inflammatory demyelinating diseases of the central nervous system.
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DOI：
10.1038/s41598-017-03370-z
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发表时间：
2017-06-09
影响因子：
4.6
通讯作者：
Hattori N
中科院分区：
文献类型：
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作者：
Cossu D;Yokoyama K;Tomizawa Y;Momotani E;Hattori N
鸟分枝杆菌副结核分枝杆菌（MAP）和牛分枝杆菌（BCG）与几种人类自身免疫性疾病如多发性硬化症（MS）有关，但在这个问题上存在相互矛盾的证据。本研究的目的是评估其在日本中枢神经系统炎性脱髓鞘疾病（IDDs）患者中的作用。采用间接ELISA法检测了97例IDDs患者（包括51例MS和46例NMOSD患者）和34例健康对照者（HC）的抗分枝杆菌IgG、IgM和伊加抗体。与NMOSD患者（AUC = 0.59，p = 0.02）和HC患者（AUC = 0.67，p = 0.01）相比，MS患者的抗MAP IgG水平更高，抗MAP抗体在接受干扰素β治疗的MS患者中更普遍（OR = 11.9; p = 0.004）。在8%的MS、32%的NMOSD和18%的HC中检测到抗BCG IgG抗体，MS和NMOSD组之间的差异具有统计学显著性（AUC = 0.66，p = 0.005）。竞争实验表明，非特异性IgM的共同分枝杆菌抗原引起的。我们的研究为MAP和MS之间可能的关联提供了进一步的证据，而BCG疫苗接种似乎与MS的风险呈负相关。
Mycobacterium avium subsp. paratuberculosis (MAP) and Mycobacterium bovis (BCG) have been associated to several human autoimmune diseases such as multiple sclerosis (MS), but there are conflicting evidence on the issue. The objective of this study is to evaluate their role in Japanese patients affected by inflammatory demyelinating disorders of the central nervous system (IDDs). A total of 97 IDDs subjects including 51 MS and 46 neuromyelitis optica spectrum disorder (NMOSD) patients, and 34 healthy controls (HCs) were tested for the detection of IgG, IgM and IgA against mycobacterial antigens by indirect ELISA. The levels of anti-MAP IgG were higher in MS patients compared to NMOSD patients (AUC = 0.59, p = 0.02) and HCs (AUC = 0.67, p = 0.01), and the anti-MAP antibodies were more prevalent in MS patients treated with interferon-beta (OR = 11.9; p = 0.004). Anti-BCG IgG antibodies were detected in 8% of MS, 32% of NMOSD and 18% of HCs, the difference between MS and NMOSD groups was statistically significant (AUC = 0.66, p = 0.005). Competition experiments showed that nonspecific IgM were elicited by common mycobacterial antigens. Our study provided further evidence for a possible association between MAP and MS, while BCG vaccination seemed to be inversely related to the risk of developing MS.