Toward reliable algorithmic self-assembly of DNA tiles: A fixed-width cellular automaton pattern
Toward reliable algorithmic self-assembly of DNA tiles: A fixed-width cellular automaton pattern
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DOI：
10.1021/nl0722830
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发表时间：
2008-07-01
期刊：
影响因子：
10.8
通讯作者：
Murata, Satoshi
中科院分区：
文献类型：
--
作者：
Fujibayashi, Kenichi;Hariadi, Rizal;Murata, Satoshi
自下而上制造纳米级结构依赖于直接自组装的化学过程。一锅反应可以达到的复杂性、精确度和产量受到我们将组装指令编码到分子本身的能力的限制。核酸为研究这些问题提供了一个平台，因为分子结构和分子内相互作用可以编码生长规律。在这里，我们使用DNA瓷砖和DNA折纸来生长包含细胞自动机图案的晶体。在一锅退火反应中，250条DNA链首先组装成一组10种自由瓷砖类型和种子结构，然后根据自动机规则从种子按算法生长。在我们的实验中，晶体生长到类似300纳米长，包含类似300块瓷砖，初始组装错误率类似于每块瓷砖1.4%。这项工作提供了证据，证明可编程的分子自组装可能足以在一锅反应中创建广泛的复杂对象。
Bottom-up fabrication of nanoscale structures relies on chemical processes to direct, self-assembly. The complexity, precision, and yield achievable by a one-pot reaction are limited by our ability to encode assembly instructions into the molecules themselves. Nucleic acids provide a platform for investigating these issues, as molecular structure and intramolecular interactions can encode growth rules. Here, we use DNA tiles and DNA origami to grow crystals containing a cellular automaton pattern. In a one-pot annealing reaction, 250 DNA strands first assemble into a set of 10 free tile types and a seed structure, then the free tiles grow algorithmically from the seed according to the automaton rules. In our experiments, crystals grew to similar to 300 nm long, containing similar to 300 tiles with an initial assembly error rate of similar to 1.4% per tile. This work provides evidence that programmable molecular self-assembly may be sufficient to create a wide range of complex objects in one-pot reactions.