Fabrication of water dispersible and biocompatible AIE-active fluorescent polymeric nanoparticles through a %26quot;one-pot%26quot; Mannich reaction
Fabrication of water dispersible and biocompatible AIE-active fluorescent polymeric nanoparticles through a %26quot;one-pot%26quot; Mannich reaction
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通过“一锅”曼尼希反应制备水分散性和生物相容性 AIE 活性荧光聚合物纳米颗粒
DOI：
10.1039/c7py00884h
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发表时间：
2017
影响因子：
4.6
通讯作者：
Wei Yen
中科院分区：
文献类型：
--
作者：
Zeng Guangjian;Liu Meiying;Jiang Ruming;Huang Qiang;Huang Long;Wan Qing;Dai Yanfeng;Wen Yuanqing;Zhang Xiaoyong;Wei Yen
荧光纳米颗粒在化学、材料、医学和生物学等领域受到越来越多科学家的关注。其中，具有聚集诱导发射（AIE）特性的荧光纳米粒子在生物医学领域的应用前景最为广阔。在这项工作中，我们首次报道了基于Mannich反应可以很容易地制备含有疏水AIE染料和亲水聚乙二醇（PEG）片段的两亲性聚合物，该聚合物可以在一个锅中偶联含酚羟基AIE染料（pheoh），多聚甲醛和4臂聚乙二醇胺（4臂PEG- nh2）。通过一系列表征技术确定了所获得的PEG- phe荧光聚合物纳米颗粒（fpn）。此外，还研究了PEG-PhE FPNs的细胞活力和细胞摄取行为，以评估其生物医学应用潜力。结果表明，PEG-PhE FPNs在生物医学领域具有巨大的应用潜力。更重要的是，这项工作中使用的策略也可以扩展到用不同的功能聚合物制备许多其他各种aie活性fpn。
Fluorescent nanoparticles have been attracting more and more interest from scientists in chemistry, materials, medical and biology fields. Among them, fluorescent nanoparticles with aggregation-induced emission (AIE) character stand out as the most promising candidates for different applications especially in the biomedical field. In this work, we reported for the first time that amphiphilic polymers containing a hydrophobic AIE dye and a hydrophilic poly(ethylene glycol) (PEG) segment can be facilely fabricated based on a Mannich reaction, which could conjugate the phenol hydroxyl-containing AIE dye (PhE-OH), paraformaldehyde and 4-arm poly(ethylene glycol)amine (4-arm PEG-NH2) in one pot. The obtained PEG-PhE fluorescent polymeric nanoparticles (FPNs) were ascertained by a series of characterization techniques. Moreover, the cell viability and cell uptake behavior of PEG-PhE FPNs were also investigated to evaluate their potential for biomedical applications. The results suggest that the PEG-PhE FPNs show great potential application in the field of biomedical science. More importantly, the strategy used in this work can also be extended for the preparation of many other various AIE-active FPNs with different functional polymers.