Relationship of mitochondrial alterations and 99mTc pyrophosphate uptake during myocardial ischemia.
Relationship of mitochondrial alterations and 99mTc pyrophosphate uptake during myocardial ischemia.
复制标题
心肌缺血期间线粒体改变与 99mTc 焦磷酸摄取的关系。
DOI：
10.1152/ajpheart.1982.243.2.h268
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Willerson,JT
中科院分区：
文献类型：
--
作者：
Mukherjee,A;Buja,LM;Kulkarni,P;Nicar,M;Chien,KR;Willerson,JT
方法选用SD大鼠，雌雄各半，体重30~35 kg。用戊巴比妥钠(30 mg/kg)静脉注射麻醉，插管，并使用哈佛呼吸器(哈佛仪器，马萨诸塞州米利斯)进行氧气呼吸。行左开胸手术，切开心包，分离冠状动脉左前降支(LAD)近端。结扎左前降支近端约1/3~1/2距离其起始点至心尖部及第一斜角支远端，以形成累及约50%的左前室(LV)的剧烈发紫区域。犬冠状动脉结扎15min(n=3)、1h(n=14)和24 h(n=6)。在这些犬中，松开结扎，注射5-15mCI*“的TC-PPI，并允许再流1.5h，另一组犬(n=4)进行15min的永久性冠状动脉闭塞而不再通。处死犬后，取出心脏，在冰冷缓冲液中快速将左室缺血组织(紫杉区)和对照组织分成心内膜下和心外膜下两半[缓冲液I：220 mM甘露醇，70 mM蔗糖，
METHODSDogs (30-35 kg) of either sex were used. The dogs were anesthetized with pentobarbital sodium (30 mg/kg iv), intubated, and ventilated with oxygen on a Harvard respirator (Harvard Apparatus, Millis, MA). A left thoracotomy was performed, the pericardium was opened, and the proximal left anterior descending coronary artery (LAD) was isolated. The proximal LAD was ligated approximately one-third to one-half the distance from its origin to the apex and distal to the first diagonal branch so as to create a region of intense cyanosis involving approximately 50% of the anterior left ventricle (LV). Dogs were subjected to coronary occlusion for 15 min (n= 3), 1 h (n= 14), or 24 h (n= 6). In these dogs the ligature ‘was released, 5-15 mCi*“Tc-PPi was injected, and reflow was allowed for 1.5 h. An additional group of dogs (n= 4) had 15 min of permanent coronary occlusion without reperfusion. After the dogs were killed, the heart was removed and the LV ischemic tissue (the cyanotic region)(13) and control tissue were divided quickly into subendocardial and subepicardial halves in ice-cold buffer [“buffer I”: 220 mM mannitol, 70 mM sucrose,