Detection of norovirus virus-like particles using a surface plasmon resonance-assisted fluoroimmunosensor optimized for quantum dot fluorescent labels
Detection of norovirus virus-like particles using a surface plasmon resonance-assisted fluoroimmunosensor optimized for quantum dot fluorescent labels
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DOI：
10.1016/j.bios.2016.08.099
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发表时间：
2017-07-15
影响因子：
12.6
通讯作者：
Fujimaki, Makoto
中科院分区：
文献类型：
--
作者：
Ashiba, Hiroki;Sugiyama, Yuki;Fujimaki, Makoto
需要一种高灵敏度的生物传感器来检测环境中的诺如病毒，以防止感染的传播。在这项研究中，我们研究了一种表面等离子体共振（SPR）辅助荧光免疫传感器的设计，以提高其灵敏度，并进行检测诺如病毒病毒样颗粒（VLP）。采用量子点荧光染料，因为其大的斯托克斯位移。对基于CdSe-ZnS量子点的传感器设计进行了优化。最佳的设计被应用到一个简单的SPR辅助荧光免疫传感器，使用传感器芯片配备了V形沟槽。量子点的激发效率，SPR的电场增强程度，和传感器芯片的基板的自发荧光的强度进行了理论和实验评估，以最大化的信噪比。结果，选择390 nm的激发波长来激发传感器芯片的Al膜上的SPR。使用所设计的传感器进行诺如病毒VLP的夹心测定。证明了0.01 ng/mL的最小可检测浓度，相当于V型沟槽检测区域中包含的100个病毒样颗粒。(C)2016年6月，作者。由爱思唯尔公司出版
A highly sensitive biosensor to detect norovirus in environment is desired to prevent the spread of infection. In this study, we investigated a design of surface plasmon resonance (SPR)-assisted fluoroimmunosensor to increase its sensitivity and performed detection of norovirus virus-like particles (VLPs). A quantum dot fluorescent dye was employed because of its large Stokes shift. The sensor design was optimized for the CdSe-ZnS-based quantum dots. The optimal design was applied to a simple SPR-assisted fluoroimmunosensor that uses a sensor chip equipped with a V-shaped trench. Excitation efficiency of the quantum dots, degree of electric field enhancement by SPR, and intensity of auto fluorescence of a substrate of the sensor chip were theoretically and experimentally evaluated to maximize the signal-to-noise ratio. As the result, an excitation wavelength of 390 nm was selected to excite SPR on an Al film of the sensor chip. The sandwich assay of norovirus VLPs was performed using the designed sensor. Minimum detectable concentration of 0.01 ng/mL, which corresponds to 100 virus-like particles included in the detection region of the V-trench, was demonstrated. (C) 2016 The Authors. Published by Elsevier B.V.