Characterization of a thrombomodulin cDNA reveals structural similarity to the low density lipoprotein receptor.
Characterization of a thrombomodulin cDNA reveals structural similarity to the low density lipoprotein receptor.
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血栓调节蛋白 cDNA 的表征揭示了其与低密度脂蛋白受体的结构相似性。
DOI：
10.1073/pnas.83.23.8834
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发表时间：
1986
影响因子：
11.1
通讯作者：
Rosenberg,RD
中科院分区：
文献类型：
--
作者：
Jackman,RW;Beeler,DL;VanDeWater,L;Rosenberg,RD
我们已经从λ gt 11牛肾上腺毛细血管内皮细胞表达文库中分离出牛血栓调节蛋白的部分长度cDNA。这是通过用兔抗血栓调节蛋白IgG异源抗体进行免疫筛选，然后用初始阳性重组插入物进行再筛选来完成的。通过显示其编码牛血栓调节蛋白的两个独立区域的一级结构来鉴定所获得的cDNA。最大的cDNA的核苷酸序列允许我们建立约80%的成熟血栓调节蛋白转录本的结构，其编码多肽的C-末端的一半。这种膜组分在结构上类似于包被凹坑受体，并被组织成类似于低密度脂蛋白受体的结构域。
We have isolated a partial-length cDNA for bovine thrombomodulin from a lambda gt11 bovine adrenal capillary endothelial cell expression library. This was accomplished by immunoscreening with rabbit anti-thrombomodulin IgG heteroantibody and then rescreening with the initial positive recombinant insert. The cDNA obtained was authenticated by showing that it coded for the primary structure of two separate regions of bovine thrombomodulin. The nucleotide sequence of the largest cDNA allowed us to establish the structure of about 80% of the mature thrombomodulin transcript, which encodes the C-terminal half of the polypeptide. This membrane component is structurally similar to coated-pit receptors and is organized into domains that resemble those of the low density lipoprotein receptor.