Effects of nitric oxide on force-generating proteins of skeletal muscle
Effects of nitric oxide on force-generating proteins of skeletal muscle
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DOI：
10.1007/s004240050391
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发表时间：
1997-07-01
影响因子：
4.5
通讯作者：
Gobesberger, A
中科院分区：
文献类型：
--
作者：
Galler, S;Hilber, K;Gobesberger, A
一氧化氮（NO）最近被鉴定为调节骨骼肌收缩活性的生理学上重要的细胞内信使[Kobzik L，Reid MB，Bredt DS，Stamler JS（1994）Nature 372：546-548]。然而，NO的作用机制尚不清楚，我们使用带皮（脱膜）的肌纤维来研究NO在肌肉收缩中的作用机制。最大钙激活的大鼠骨骼肌单纤维暴露于生理相关的NO浓度通过添加NO供体分子到浴溶液中。供体应用引起的收缩性能和肌原纤维腺苷三磷酸酶（ATP酶）的活性下降。这些结果揭示了一种新的NO作用的分子机制：直接抑制骨骼肌中的力产生蛋白。
Nitric oxide (NO) has recently been identified as a physiologically important intracellular messenger modulating the contractile activity of skeletal muscle [Kobzik L, Reid MB, Bredt DS, Stamler JS (1994) Nature 372: 546-548]. However, the mechanism of action of NO is not yet known, We used skinned (demembranated) muscle fibres to investigate the mechanism of NO function in muscle contraction. Maximally Ca2+-activated single fibres of rat skeletal muscle were exposed to physiologically relevant NO concentrations by adding NO donor molecules into the bath solution. Donor application caused a decline both in the contractile properties and in the myofibrillar adenosine triphosphatase (ATPase) activity. These results reveal a novel molecular mechanism of NO action: a direct inhibition of the force-generating proteins in skeletal muscle.