COMPLETE PRIMARY STRUCTURES OF THE E-BETA CHAIN AND GENE OF THE MOUSE MAJOR HISTOCOMPATIBILITY COMPLEX
COMPLETE PRIMARY STRUCTURES OF THE E-BETA CHAIN AND GENE OF THE MOUSE MAJOR HISTOCOMPATIBILITY COMPLEX
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DOI：
10.1073/pnas.80.18.5520
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
TONEGAWA, S
中科院分区：
文献类型：
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作者：
SAITO, H;MAKI, RA;TONEGAWA, S
使用与质粒pDC β- 1，含有编码DC β的c[互补]DNA。人主要组织相容性复合体II类分子的cDNA链，E β的cDNA和基因组基因。克隆BALB/c（d单倍型）小鼠的DNA序列并进行序列分析。cDNA和基因组DNA克隆的核苷酸序列允许推导E β的整个一级结构。链和E β的完整外显子-内含子结构。基因与α不同。链基因，包含5个外显子，E β。基因由6个外显子组成，对应于6个功能结构域：前导区、β 1和β 2结构域、跨膜肽、胞质内肽和3 ″非翻译区。两个短的序列块共同的α。和β在5“侧翼区也鉴定了链基因。这些序列参与α的配位表达。和β店
Using the cross-hybridization with plasmid pDC.beta.-1, containing the c[complementary]DNA coding for the DC.beta. chain of the human major histocompatibility complex class II molecules, both the cDNA and genomic gene for the E.beta. chain of the BALB/c (d haplotype) mouse were cloned and subjected to sequence analysis. The nucleotide sequences of the cDNA and genomic DNA clones permitted deduction of the entire primary structure of the E.beta. chain and the complete exon-intron structure of the E.beta. gene. Unlike .alpha. chain genes that contain 5 exons, the E.beta. gene consists of 6 exons corresponding to the 6 functional domains: the leader, .beta.1 and .beta.2 domains, transmembrane peptide, intracytoplasmic peptide and 3'' untranslated region. Two short blocks of sequences common to .alpha. and .beta. chain genes were also identified in the 5'' flanking regions. These sequences are involved in the coordinate expression of .alpha. and .beta. chains.