Mechanisms of sex differentiation and sex reversal in hermaphrodite fish as revealed by the Epinephelus coioides genome
Mechanisms of sex differentiation and sex reversal in hermaphrodite fish as revealed by the Epinephelus coioides genome
复制标题
斜带石斑鱼基因组揭示雌雄同体鱼类性别分化和性别逆转的机制
DOI：
10.1111/1755-0998.13753
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发表时间：
2023-01-19
影响因子：
7.7
通讯作者：
Zhang，Yong
中科院分区：
文献类型：
--
作者：
Li，Shuisheng;Li，Wanshun;Zhang，Yong
大多数石斑鱼物种是功能性的先雌先孕雌雄同体，但这种独特的生殖策略的遗传基础和分子机制的调节仍然是谜。在这项研究中，我们报告了一个高质量的染色体水平的基因组组装的代表性橙子斑点石斑鱼（Epinealphus coioides）。在基因组中没有发现性别分化相关基因的重复或缺失，这表明该石斑鱼的性别发育可能与调控序列或环境因素的变化有关。转录组学分析表明，芳香化酶和视黄酸可能对促进卵巢命运决定至关重要，而卵泡刺激素触发了女性到男性的性别转变。社会控制的性别改变研究表明，在性别改变的鱼类中，大脑对社会环境的反应可能是通过激活光转导级联和褪黑激素合成途径来介导的。总之，我们的基因组和实验结果提供了新的见解的分子机制的性别分化和性别变化的先雌石斑鱼。
Most grouper species are functional protogynous hermaphrodites, but the genetic basis and the molecular mechanisms underlying the regulation of this unique reproductive strategy remain enigmatic. In this study, we report a high‐quality chromosome‐level genome assembly of the representative orange‐spotted grouper (Epinephelus coioides). No duplication or deletion of sex differentiation‐related genes was found in the genome, suggesting that sex development in this grouper may be related to changes in regulatory sequences or environmental factors. Transcriptomic analyses showed that aromatase and retinoic acid are probably critical to promoting ovarian fate determination, and follicle‐stimulating hormone triggers the female‐to‐male sex change. Socially controlled sex‐change studies revealed that, in sex‐changing fish, the brain's response to the social environment may be mediated by activation of the phototransduction cascade and the melatonin synthesis pathway. In summary, our genomic and experimental results provide novel insights into the molecular mechanisms of sex differentiation and sex change in the protogynous groupers.