GoIS controls the response to gold by the hierarchical induction of Salmonella-specific genes that include a CBA efflux-coding operon
GoIS controls the response to gold by the hierarchical induction of Salmonella-specific genes that include a CBA efflux-coding operon
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DOI：
10.1111/j.1365-2958.2007.05963.x
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发表时间：
2007-11-01
影响因子：
3.6
通讯作者：
Soncini, Fernando C.
中科院分区：
文献类型：
--
作者：
Pontel, Lucas B.;Audero, Maria E. Perez;Soncini, Fernando C.
沙门氏菌使用一组特定的蛋白质，使其能够检测环境中金盐的存在并产生适当的抵抗反应。这包括P型ATP酶，GoIT和小的细胞质金属结合蛋白，GoIB。它们的表达由MerR样传感器GoIS控制，GoIS对Au离子具有高度选择性。在这里，我们确定了一个新的GoIS控制的操纵子命名为gesABC的CBA外排系统的代码，并建立其在Au电阻的作用。GesABC也可以介导药物耐药性时，诱导Au在GoIS依赖性的方式，在一个菌株中删除的主要药物转运蛋白acrAB。GoIS调控的gesABC转录与其他GoIS调控的基因座不同。它被金激活，但不被铜诱导，即使在缺失主要铜转运蛋白基因copA的菌株中，其触发goITS和goIB的大量GoIS依赖性诱导。我们表明，金依赖诱导gesABC转录需要更高的GoIS水平比其他成员的gol调节子。这与gesABC启动子中的趋异GoIS操纵子相关。我们建议，层次内的gol调节子的诱导，使沙门氏菌，以科普金污染的环境。
Salmonella employs a specific set of proteins that allows it to detect the presence of gold salts in the environment and to mount the appropriate resistance response. This includes a P-type ATPase, GoIT, and a small cytoplasmic metal binding protein, GoIB. Their expression is controlled by a MerR-like sensor, GoIS, which is highly selective for Au ions. Here, we identify a new GoIS-controlled operon named gesABC which codes for a CBA efflux system, and establish its role in Au resistance. GesABC can also mediate drug resistance when induced by Au in a GoIS-dependent manner, in a strain deleted in the main drug transporter acrAB. The GoIS-controlled transcription of gesABC differs from the other GoIS-regulated loci. It is activated by gold, but not induced by copper, even in a strain deleted of the main Cu transporter gene copA, which triggers a substantial GoIS-dependent induction of goITS and goIB. We demonstrate that the Au-dependent induction of gesABC transcription requires higher GoIS levels than for the other members of the gol regulon. This correlates with a divergent GoIS operator in the gesABC promoter. We propose that the hierarchical induction within the gol regulon allows Salmonella to cope with Au-contaminated environments.