Neuroprotective effect of calycosin on cerebral ischemia and reperfusion injury in rats
Neuroprotective effect of calycosin on cerebral ischemia and reperfusion injury in rats
复制标题
毛蕊异黄酮对大鼠脑缺血再灌注损伤的神经保护作用。
DOI：
10.1016/j.jep.2012.09.056
复制
发表时间：
2012-12-18
影响因子：
5.4
通讯作者：
Wen, Aidong
中科院分区：
文献类型：
--
作者：
Guo, Chao;Tong, Li;Wen, Aidong
民族药理学相关性：黄芪是我国常用的传统中药，具有补气固表、排脓生新的作用。研究目的：本研究旨在探讨从黄芪根中提取的毛蕊异黄酮素对脑缺血/再灌注损伤的神经保护作用。 大脑中动脉闭塞（MCAO）诱导缺血2小时后再灌注24小时，给Sprague-Dawley大鼠灌胃不同剂量的毛蕊异黄酮（分别为7.5、15、30mg/kg）。再灌注24小时后评估神经功能缺损、梗塞体积、组织病理学变化和一些氧化应激标志物。结果：毛蕊异黄酮治疗显着改善脑缺血再灌注后的神经功能缺损和梗塞体积。毛蕊异黄酮还可以降低丙二醛（MDA）、蛋白质羰基和活性氧（ROS）的含量，并以剂量依赖性方式上调超氧化物歧化酶（SOD）、过氧化氢酶和谷胱甘肽过氧化物酶（GSH-Px）的活性。此外，毛蕊异黄酮还能抑制4-羟基-2-壬烯醛(4-HNE)的表达。结论：这些结果表明毛蕊异黄酮对脑缺血/再灌注损伤具有神经保护作用。该机制可能归因于其抗氧化作用。 (C) 2012 Elsevier Ireland Ltd. 保留所有权利。
Ethnopharmacological relevance: Radix Astragali has been commonly used as traditional herbal medicine in China for reinforcing vital energy, strengthening superficial resistance and promoting the discharge of pus and the growth of new tissue.Aim of the study: The present study was to investigate the neuroprotective effect of calycosin isolated from the roots of Radix Astragali on cerebral ischemic/reperfusion injury.Materials and methods: After 24 h of reperfusion following ischemia for 2 h induced by middle cerebral artery occlusion (MCAO), Sprague-Dawley rats were intragastrically administered different doses of calycosin (7.5, 15, 30 mg/kg, respectively). Neurological deficit, infarct volume, histopathology changes and some oxidative stress markers were evaluated after 24 h of reperfusion.Results: Treatment with calycosin significantly ameliorated neurologic deficit and infarct volume after cerebral ischemia reperfusion. Calycosin also reduced the content of malondialdehyde (MDA), protein carbonyl and reactive oxygen species (ROS), and up-regulated the activities of superoxide dismutase (SOD), catalase and glutathione peroxidase (GSH-Px) in a dose-dependent manner. Moreover, calycosin can also inhibit the expression of 4-Hydroxy-2-nonenal (4-HNE).Conclusion: These results suggest that calycosin has a neuroprotective effect against cerebral ischemia/reperfusion injury. The mechanism might be attributed to its antioxidant effects. (C) 2012 Elsevier Ireland Ltd. All rights reserved.