Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester
Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester
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DOI：
10.1038/nprot.2007.296
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发表时间：
2007-01-01
期刊：
影响因子：
14.8
通讯作者：
Parish, Christopher R.
中科院分区：
文献类型：
--
作者：
Quah, Ben J. C.;Warren, Hilary S.;Parish, Christopher R.
该协议概述了羧基荧光素二乙酸琥珀酰亚胺酯 (CFSE) 方法，用于跟踪体外人类淋巴细胞和体外和体内小鼠淋巴细胞的增殖。该方法依赖于 CFSE 用高荧光染料羧基荧光素共价标记长寿命细胞内分子的能力。每次细胞分裂后，这些荧光分子平均分配给子代细胞，导致子代细胞的荧光减半。所描述的 CFSE 标签协议，通常需要
This protocol outlines the carboxyfluorescein diacetate succinimidyl ester (CFSE) method for following the proliferation of human lymphocytes in vitro and mouse lymphocytes both in vitro and in vivo. The method relies on the ability of CFSE to covalently label long-lived intracellular molecules with the highly fluorescent dye, carboxyfluorescein. Following each cell division, the equal distribution of these fluorescent molecules to progeny cells results in a halving of the fluorescence of daughter cells. The CFSE labeling protocol described, which typically takes