[The effect of mangiferin on telomerase activity and apoptosis in leukemic K562 cells].
[The effect of mangiferin on telomerase activity and apoptosis in leukemic K562 cells].
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发表时间：
2007-03
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影响因子：
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通讯作者：
P. Cheng;Zhi-gang Peng;Jie Yang;Shan-jun Song
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作者：
P. Cheng;Zhi-gang Peng;Jie Yang;Shan-jun Song
目的观察芒果苷对K562细胞株端粒酶活性和细胞凋亡的影响，探讨其抗白血病的分子机制。方法光镜和透射电镜观察K562细胞凋亡情况;流式细胞术检测Fas表达;聚合酶链反应-酶联免疫法（PCR-ELISA）检测端粒酶活性。结果芒果苷对K562细胞端粒酶活性有抑制作用，并呈时间和浓度依赖性。同时，能明显诱导K562细胞凋亡，上调Fas水平。结论芒果苷可抑制K562细胞端粒酶活性，其作用机制可能与诱导细胞凋亡及Fas蛋白表达有关。
OBJECTIVE To investigate the effects of mangiferin on telomerase activity and apoptosis in K562 cells lines and study the melocular mechanism of it's antileukemic. METHODS Cell apoptosis was observed by light microscopy and transmission electron microscopy; The expression of Fas was measured by flow cytometry; Polymerase chain reaction enzyme linked immunoassay (PCR-ELISA) was used to assay telomerase activity of K562 cells. RESULTS Mangiferin could inhibit telomerase activity of K562 cells in a time-and-concentration-dependent manner. Meanwhile, it could induce apoptosis obviously and up-regulate the levels of Fas in K562 cells. CONCLUSION Mangiferin can inhibit telomerase activity of K562 cells, and the mechanism of effect is maybe relate to inducing apoptosis and the expression of Fas protein.