An investigation on the analytical potential of polymerized liposomes bound to lanthanide ions for protein analysis.
An investigation on the analytical potential of polymerized liposomes bound to lanthanide ions for protein analysis.
复制标题
研究了与镧系离子结合的聚合脂质体用于蛋白质分析的分析潜力。
DOI：
10.1021/ja048963b
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Mallik,Sanku
中科院分区：
文献类型：
--
作者：
Santos,Marina;Roy,BidhanC;Goicoechea,Hector;Campiglia,AndresD;Mallik,Sanku
We present a promising approach to protein sensing based on Eu3+ions incorporated into polymerized liposomes. The sensitization of Eu3+is accomplished with 5-aminosalicylic acid, which provides energy transfer for a stable reference signal and a wide wavelength excitation range free from protein interference. The lipophilic character of polymerized liposomes provides the appropriate platform for protein interaction with the lanthanide ion. Quantitative analysis is based on the linear relationship between the luminescence signal of Eu3+and protein concentration. Because no spectral shift of the lanthanide luminescence is observed upon protein interaction, qualitative analysis is based on the luminescence lifetime of polymerized liposomes. This parameter, which changes significantly upon protein−liposome interaction, follows a well-behaved single-exponential decay that might be useful for protein identification.
