SYMPATHETIC REGULATION OF RENAL FUNCTION IN STROKE-PRONE SPONTANEOUSLY HYPERTENSIVE RATS CONGENIC FOR CHROMOSOME 1 BLOOD PRESSURE QUANTITATIVE TRAIT LOCI
SYMPATHETIC REGULATION OF RENAL FUNCTION IN STROKE-PRONE SPONTANEOUSLY HYPERTENSIVE RATS CONGENIC FOR CHROMOSOME 1 BLOOD PRESSURE QUANTITATIVE TRAIT LOCI
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DOI：
10.1111/j.1440-1681.2008.04990.x
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发表时间：
2008-11-01
影响因子：
2.9
通讯作者：
Nabika, Toru
中科院分区：
文献类型：
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作者：
Wang, Tao;Takabatake, Toshikazu;Nabika, Toru
1.通过将卒中易感自发性高血压大鼠(SHRSP)衍生的1号染色体血压(BP)数量性状基因座(QTL)片段导入Wistar-京都(WKY)大鼠，构建了WKYpch1.0和SHRSPwch1.0两个相互同源的品系。在有完整肾交感神经的基础条件下，肾去甲肾上腺素含量和肾血管阻力(RVR)按SHRSP、SHRSPwch1.0、WKYpch1.0和WKY的顺序依次降低，并根据1号染色体QTL.3基因的不同呈现出相互变化的关系。去肾神经只对SHRSP和WKYpch1.0的RVR和部分钠排泄有显著影响，这两个区域都含有SHRSP衍生的1号染色体QTL.4片段。因此，1号染色体QTL可能既影响肾交感神经活动，也影响交感神经系统对血管和肾小管功能的调节作用。因此，相互同源的菌株是独特的模式，可能有助于寻找中间表型，并使候选基因的功能推断成为可能。
1. Two reciprocal congenic strains, WKYpch1.0 and SHRSPwch1.0, were constructed, respectively, by introgressing the stroke-prone spontaneously hypertensive rat (SHRSP)-derived fragment for the chromosome 1 blood pressure (BP) quantitative trait locus (QTL) into Wistar-Kyoto (WKY) rats and vice versa.2. Under basal conditions with intact renal sympathetic nerves, the renal noradrenaline content and renal vascular resistance (RVR) were decreased in the order of SHRSP, SHRSPwch1.0, WKYpch1.0 and WKY, exhibiting reciprocal changes in the congenic strains according to the genotype of the chromosome 1 QTL.3. Renal denervation resulted in significant effects on RVR and the fractional excretion of sodium only in SHRSP and WKYpch1.0, both of which harboured the SHRSP-derived fragment of chromosome 1 QTL.4. Thus, chromosome 1 QTL may influence both renal sympathetic nervous activity and the regulatory role of the sympathetic nervous system in vascular and tubular functions. The reciprocal congenic strains are thereby unique models that may help in the search for intermediate phenotypes and empower functional deduction of candidate genes.