A subpopulation of mouse esophageal basal cells has properties of stem cells with the capacity for self-renewal and lineage specification
A subpopulation of mouse esophageal basal cells has properties of stem cells with the capacity for self-renewal and lineage specification
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DOI：
10.1172/jci35012
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发表时间：
2008-12-01
影响因子：
15.9
通讯作者：
Rustgi, Anil K.
中科院分区：
文献类型：
--
作者：
Kalabis, Jiri;Oyama, Kenji;Rustgi, Anil K.
食管上皮是典型的复层鳞状上皮，其在增殖和分化之间表现出精致的平衡。基底细胞增殖后，它们向外迁移到管腔表面，经历分化，并最终由于凋亡而脱落斯劳。负责维持食管上皮的干细胞的鉴定和表征仍然难以捉摸。在这里，我们采用Hoechst染料挤出和BrdU标记保留试验，以确定在小鼠中的一个潜在的食管干细胞群体，定位于基底细胞室。该群体的自我更新能力的特点是使用克隆形成试验和三维器官型培养模型。推测的食管干细胞也能够上皮化。在直接食管上皮损伤模型中的体内重建。在3D器官型培养和直接粘膜损伤模型中，假定的干细胞产生未分化和分化的细胞。因此，这些研究提供了一个基础，了解再生能力和生物学的食管上皮细胞时，它面临着有害的侮辱。
The esophageal epithelium is a prototypical stratified squamous epithelium that exhibits an exquisite equilibrium between proliferation and differentiation. After basal cells proliferate, they migrate outward toward the luminal surface, undergo differentiation, and eventually slough due to apoptosis. The identification and characterization of stem cells responsible for the maintenance of the esophageal epithelium remains elusive. Here, we employed Hoechst dye extrusion and BrdU label-retaining assays to identify in mice a potential esophageal stem cell population that localizes to the basal cell compartment. The self-renewing capacity of this population was characterized using a clonogenic assay and a 3D organotypic culture model. The putative esophageal stem cells were also capable of epithelial. reconstitution in vivo in direct esophageal epithelial injury models. In both the 3D organotypic culture and direct mucosal injury models, the putative stem cells gave rise to undifferentiated and differentiated cells. These studies therefore provide a basis for understanding the regenerative capacity and biology of the esophageal epithelium when it is faced with injurious insults.