Increased subventricular zone-derived cortical neurogenesis after ischemic lesion
Increased subventricular zone-derived cortical neurogenesis after ischemic lesion
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DOI：
10.1016/j.expneurol.2010.08.006
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发表时间：
2010-11-01
影响因子：
5.3
通讯作者：
Khodosevich, Konstantin
中科院分区：
文献类型：
--
作者：
Kreuzberg, Maria;Kanov, Evgeny;Khodosevich, Konstantin
在成年啮齿动物中，中风促进了神经发生，导致神经元增加到前脑区域，如纹状体或皮质，在正常情况下，出生后的神经发生在这些区域起到的作用可以忽略不计。在大脑皮层，新的神经元要么来自中风激活的局部皮质前体，要么来自驻留在侧脑室室下区(SVZ)的前体，在正常情况下，这些前体基本上只为嗅球提供神经母细胞。在这项研究中，我们使用了5HT3A-EGFP转基因小鼠，在这些小鼠中，所有起源于SVZ的神经母细胞都被EGFP标记。我们在这些小鼠中诱导了卒中，通过结合EGFP检测和BrdU注射，我们标记了所有卒中后产生的SVZ来源的神经母细胞。我们发现卒中后14天和35天，同侧大脑半球SVZ来源的神经母细胞增多。卒中后SVZ来源的神经母细胞迁移到皮质并存活至少35天，占周围梗死区BrdU阳性细胞的2%，在那里它们分化为成熟神经元。因此，中风促进了SVZ的神经发生，并将新生神经元吸引到损伤区域。(C)2010 Elsevier Inc.保留所有权利。
In adult rodents stroke enhances neurogenesis resulting in the addition of neurons to forebrain regions such as striatum or cortex where postnatal neurogenesis under normal conditions plays a negligible role. In the cortex, new neurons are generated either from local cortical precursors that are activated by stroke or from precursors residing in the subventricular zone (SVZ) of lateral ventricles that under normal conditions supply neuroblasts by and large only for the olfactory bulb. In this study we used 5HT3A-EGFP transgenic mice in which all neuroblasts originating in the SVZ are EGFP-labeled. We induced stroke in these mice and by combination of EGFP detection with BrdU injections we labeled all post-stroke-generated SVZ-derived neuroblasts. We showed an increase in SVZ-derived neuroblasts 14 and 35 days after stroke in the ipsilateral hemisphere. Post-stroke-generated SVZ-derived neuroblasts migrated to the cortex and survived for at least 35 days representing 2% of BrdU-positive cells in pen-infarct area where they differentiate into mature neurons. Thus, stroke enhances SVZ neurogenesis and attracts newborn neurons to the injury zone. (C) 2010 Elsevier Inc. All rights reserved.