Leptin: Clue to poor appetite in oxygen-starved fish
Leptin: Clue to poor appetite in oxygen-starved fish
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DOI：
10.1016/j.mce.2010.01.018
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发表时间：
2010-05-05
影响因子：
4.1
通讯作者：
Yu, Richard Man Kit
中科院分区：
文献类型：
--
作者：
Chu, Daniel Ling Ho;Li, Vincent Wai Tsun;Yu, Richard Man Kit
缺氧是富营养化最普遍的有害后果，并已成为渔业衰退的主要原因。在这项研究中，我们研究了编码食欲抑制激素瘦素的基因是否受到斑马鱼（Danio rerio）缺氧的调节。成年斑马鱼暴露于低氧条件（1 +/-02mgO-2L-1）4天和10天后，肝脏leptin-a（zlep-a）mRNA水平显著升高。为了评估缺氧诱导因子1（HIF-1）在调节zlep-a表达中的作用，将斑马鱼胚胎暴露于氯化钴（CoCl 2. HIF-1诱导剂）并用HIF-1 α mRNA过表达。CoCl 2处理和HIF-1 α都显著增加了发育中胚胎中zlep-a的表达，表明HIF-1可能参与zlep-a的调节体内启动子分析表明zlep-a启动子活性存在于斑马鱼胚胎的肌纤维中，并被CoCl 2增强。这是首次报道表明鱼中瘦素基因的表达可能通过HIF-1的参与受到缺氧的调节（C）2010爱思唯尔爱尔兰有限公司保留所有权利。
Hypoxia is the most widespread deleterious consequence of eutrophication and has become a major cause of fishery decline. One feature of chronic exposure to hypoxia in fish is inhibition of feeding In this study, we investigated if the gene that encodes the appetite-suppressing hormone leptin is regulated by hypoxia in zebrafish (Danio rerio). Exposure of adult zebrafish to hypoxic conditions (1 +/- 0 2 mg O-2 L-1) for 4 and I 0 days significantly increased leptin-a (zlep-a) mRNA levels in the liver. To evaluate the role of hypoxia-inducible factor 1 (HIF-1) in regulating zlep-a expression, zebrafish embryos were exposed to cobalt chloride (CoCl2. a HIF-1 inducer) and overexpressed with HIF-1 alpha mRNA. Both CoCl2 treatment and HIF-1 alpha markedly increased zlep-a expression in developing embryos, indicating the possible involvement of HIF-1 in zlep-a regulation In vivo promoter analysis indicated that zlep-a promoter activity is found in the muscle fibers of zebrafish embryos and enhanced by CoCl2 This is the first report to show that leptin gene expression in fish is regulated by hypoxia possibly via the involvement of HIF-1 (C) 2010 Elsevier Ireland Ltd All rights reserved.