Iron oxide-transfection agent complexes are not expected to coat the cell membrane and prevent CD71 expression.
Iron oxide-transfection agent complexes are not expected to coat the cell membrane and prevent CD71 expression.
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DOI：
10.1148/radiol.2473071885
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发表时间：
2008-06
期刊：
影响因子：
19.7
通讯作者：
B. Janic;A. Arbab
中科院分区：
文献类型：
--
作者：
B. Janic;A. Arbab
摄取到细胞是预期的，尽管在各自的文章中，铁颗粒的内化尚未通过电子显微镜证实。迄今为止，我们还不能阐明导致spio介导的CD71在大鼠间充质干细胞中上调的分子机制。然而，两个完全独立的团队已经证明，用铁颗粒标记可以对干细胞生物学产生影响，这是至关重要的一点。在人类移植标记间充质干细胞后，包括安全性问题在内的功能完整性对患者至关重要，因此必须进行批判性和全方位的研究。先前的工作，特别是来自这两封信的作者，是令人鼓舞和有价值的，但对干细胞标记的相互作用的进一步研究是必要的，因为即使是像聚赖氨酸这样的TAs，也不是所有的安全问题都得到了充分的解决（5,6）。
uptake into cells is expected, although in the respective article the internalization of the iron particles has not been confirmed by means of electron microscopy. To date we can not elucidate the molecular mechanisms leading to the SPIO-mediated CD71 upregulation in rat MSCs. However, two completely independent teams have demonstrated that labeling with iron particles can have an impact on stem cell biology, which is the essential point. After transplanting labeled MSCs in humans, the integrity of functionality including issues of safety is crucial for the patients, so critical and all-embracing research is mandatory. The previous work, especially from the authors of the two letters, is encouraging and valuable, but further investigations on interactions of stem cell labeling are required, since even for TAs like poly-l-lysine not all safety concerns (5, 6) have been adequately addressed.