Use of an H-Y Antigen Assay for Sex Determination in Sea Turtles
Use of an H-Y Antigen Assay for Sex Determination in Sea Turtles
复制标题
使用 H-Y 抗原测定法确定海龟性别
DOI：
10.2307/1446036
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发表时间：
1987
期刊：
影响因子：
2.6
通讯作者：
Debra J. Wellins
中科院分区：
文献类型：
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作者：
Debra J. Wellins
采用H-Y抗原细胞毒试验对成年绿色海龟（Chelonia mydas）和红海龟（Caretta caretta）的H-Y抗原阳性性别进行了鉴定。雄性和雌性海龟血细胞（取样后小于或等于24小时测定）的特异性细胞毒性百分比在两个物种之间存在显著差异（P <0.01）。雄性海龟具有较高水平的H-Y抗原，这通过用雄性海龟血细胞预吸收的H-Y抗血清的细胞毒性降低来证明。结果与具有XX/XY性别决定机制的物种中H-Y阳性雄性的模式一致，但这尚未被海龟的核型分析证实。
An H-Y antigen cytotoxicity assay was used to determine the H-Y positive sex of adult green (Chelonia mydas) and loggerhead (Caretta caretta) sea turtles. There was a significant difference (P < .01) in the percent specific cytotoxicity between male and female sea turtle blood cells (assayed less than or equal to 24 h post sampling) for both species tested. Males had higher levels of H-Y antigen as demonstrated by a reduction in cytotoxicity of H-Y antiserum pre-absorbed with male sea turtle blood cells. The results were consistent with a pattern of H-Y positive males in species with an XX/XY sex-determining mechanism, but this has not been confirmed by karyotypic analyses in sea turtles.