Ultrastructural, physiological and proteomic analysis of Nostoc flagelliforme in response to dehydration and rehydration
Ultrastructural, physiological and proteomic analysis of Nostoc flagelliforme in response to dehydration and rehydration
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发菜对脱水和补水反应的超微结构、生理和蛋白质组学分析
DOI：
10.1016/j.jprot.2012.07.041
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发表时间：
2012-10-22
影响因子：
3.3
通讯作者：
Chen, Wei
中科院分区：
文献类型：
--
作者：
Liang, Wenyu;Zhou, Youwen;Chen, Wei
发菜必须经历一个脱水/复水周期在其生长阶段，这种约束的机制进行检查。小说对N.从超微结构、生理学和蛋白质组学等方面阐述了鞭毛藻对脱水和复水的反应。脱水和复水处理后，菌落和细胞的结构保持不变，但鞘出现皱缩，空泡的数量和体积均减少。光合作用、呼吸作用、总Rubisco活性、超氧阴离子水平、SOD、CAT、POD、固氮酶和谷氨酰胺合成酶（GS）活性均显著增加，而H2 O2、铵态氮、脯氨酸和谷氨酸含量均下降。将32个脱水和复水菌落差异表达的蛋白质按其预测功能分为分泌、信号传导、转录和翻译、抗氧化过程、氮代谢、能量代谢、脂质代谢和伴侣蛋白。脱水是一种静止状态，其中代谢被下调，在复水后，代谢从静止转变为活跃。研究了N. flagelliforme在这里报道。(C)2012爱思唯尔有限公司版权所有。
Nostoc flagelliforme must undergo a dehydration/rehydration cycle during its growth stages; the mechanisms underlying this constraint are examined. The novel insights into N. flagelliforme's response to desiccation and rehydration at ultrastructural, physiological and proteomic levels were offered. The structure of colonies and cells remained unchanged in response to dehydration and rehydration treatments except that the sheath appeared shrunken, and both the quantity and volume of vacuoles were decreased when dehydrated compared with rehydration. A significant increase in photosynthesis, respiration, total Rubisco activity, superoxide anion level, SOD, CAT, POD, nitrogenase and glutamine synthetase (GS) activities in response to rehydration was noted, whereas H2O2, ammonium, proline and glutamate contents all registered a decrease. 32 differentially expressed proteins between dehydrated and rehydrated colonies were categorized according to their predicted functions into secretion, signaling, transcription and translation, antioxidative processes, nitrogen metabolism, energy metabolism, lipid metabolism and chaperonin. The dehydration is a quiescent state in which metabolism is down-regulated, upon rehydration, a metabolic shift occurs from quiescent to active. The specific metabolic and regulated mechanisms to accommodate the dehydration/rehydration cycle in N. flagelliforme is reported here. (C) 2012 Elsevier B.V. All rights reserved.