Matrix metalloproteinase 2 and 9 expression correlated with cavernous sinus invasion of pituitary adenomas
Matrix metalloproteinase 2 and 9 expression correlated with cavernous sinus invasion of pituitary adenomas
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DOI：
10.2152/jmi.52.151
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发表时间：
2005-08-01
影响因子：
0.7
通讯作者：
Nagao, Seigo
中科院分区：
文献类型：
--
作者：
Liu, Weiping;Matsumoto, Yoshihito;Nagao, Seigo
目的：基质金属蛋白酶2和9在肿瘤侵袭过程中对组织的破坏起重要作用。本研究的目的是探讨垂体腺瘤组织中基质金属蛋白酶-2、基质金属蛋白酶-9、MIB-1LI的表达与海绵窦侵袭的关系。免疫组织化学方法检测MIB-1标记指数(LI)、基质金属蛋白酶-2(MMP2)、MMP9表达。结果：16例患者中女性34例，男性20例，年龄18~76岁，平均49.9岁。海绵窦侵犯12例，非侵袭性42例。侵袭性病例的基质金属蛋白酶-2和基质金属蛋白酶-9评分(3.9。0.5，4.1。0.4)显著高于2.3。0.2；p.0.01；2.6.0.2；p.0.01)，无侵袭。侵袭性和非侵袭性垂体腺瘤的MIB-1LI差异无统计学意义。基质金属蛋白酶-2基因的表达百分率。-肌动蛋白基因和基质金属蛋白酶-9基因的表达。-肌动蛋白mRNA在侵袭性垂体腺瘤中的表达也显著升高(68.2。15.3%；59.7%。12.5%)高于非侵袭性垂体腺瘤(21.8%)。8.2%，p.0.05；33.3。结论：我们的研究提示，在侵袭性垂体腺瘤中，基质金属蛋白酶-2和基质金属蛋白酶-9的表达可能有一定的价值，而垂体腺瘤的增殖和侵袭可能存在不同的机制。
Object: Matrix metalloproteinase (MMP) 2 and 9 are important for tissue breakdown in the process of tumor invasion. The aim of this study is to evaluate the relationship between the expression of MMP-2, MMP-9, MIB-1 LI and cavernous sinus invasion in pituitary adenomas.Methods: Tissue samples from 54 patients with pituitary adenomas were studied. Expression of MMP-2, MMP-9, and MIB-1 labeling index (LI) were evaluated by immunohistochemical method. In sixteen cases, the expression of MMP-2 and MMP-9 mRNA was also examined by RT-PCR assay.Results: Thirty-four patients were women and 20 were men, with a mean age of 49.9 years old (range 18-76 years). There were 12 cases with cavernous sinus invasion, and 42 were noninvasive cases. MMP-2 and MMP-9 score of invasive case (3.9. 0.5,4.1. 0.4) were significantly higher than those (2.3. 0.2; p. 0.01; 2.6. 0.2; p. 0.01) without invasion. The MIB-1 LI of this study presented no significantly difference between the invasive and noninvasive pituitary adenomas. The percentage of MMP-2 mRNA/. -actin mRNA and MMP-9 mRNA/. -actin mRNA were also observed significantly higher in invasive pituitary adenomas (68.2. 15.3%; 59.7. 12.5%) than noninvasive pituitary adenomas (21.8. 8.2%, p. 0.05; 33.3. 5.4%, p. 0.05).Conclusions: Our study suggests that the expression of MMP-2 and MMP-9 may have a value to assess the invasive pituitary adenomas, and proliferation and invasion of pituitary adenomas may present a different mechanism.