Immunophenotype and functional properties of feline dendritic cells derived from blood and bone marrow
Immunophenotype and functional properties of feline dendritic cells derived from blood and bone marrow
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DOI：
10.1016/s0165-2427(03)00132-6
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发表时间：
2003-11-15
影响因子：
1.8
通讯作者：
Chow, C
中科院分区：
文献类型：
--
作者：
Bienzle, D;Reggeti, F;Chow, C
树突状细胞 (DC) 是一种异质细胞群，对于启动先天性和特异性免疫反应至关重要。猫体内树突状细胞的身份和功能尚不清楚，尽管它们可能在感染反应中发挥关键作用。在这项研究中。猫 DC 是通过在 IL 4 和 GM-CSF 存在下贴壁血液单核细胞 (PBMC) 和骨髓单核细胞 (BMMC) 培养 3-10 天而获得的。 BMMC 始终比 PBMC 产生更多数量的 DC。并且两个隔室中的巨噬细胞都比 DC 少。 DC 表达一系列独特的表面分子，包括 CD1a、CD1b、CD1c、CD11b、CD14，以及比共培养巨噬细胞或新鲜血单核细胞高 2-3 倍水平的 MHC I 类和 II 类分子。 DC表现出典型的细胞质过程、有限的非特异性酯酶活性，并通过吞噬作用、胞饮作用和与特异性受体结合来获得抗原。暴露于细胞因子的细胞诱导同种异体淋巴细胞增殖。因此，通过这些培养条件衍生的细胞具有类似于未成熟骨髓DC的标记和功能。猫树突状细胞的出现将有助于研究它们在传染病中的作用及其潜在的治疗应用。 (C) 2003 Elsevier B.V. 保留所有权利。
Dendritic cells (DCs) are a heterogeneous population of cells of fundamental importance in initiating innate as well as specific immune responses. The identity and function of DCs in the cat are unknown, although they are likely pivotal in the response to infection. In this study. feline DCs were derived by 3-10-day culture of adherent blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs) in the presence of IL 4 and GM-CSF. BMMC consistently yielded a greater number of DCs than PBMC. and there were fewer macrophages than DC from both compartments. DCs expressed a distinct constellation of surface molecules, which included CD1a, CD1b, and CD1c, CD11b, CD14, and 2-3-fold higher levels of MHC class I and II molecules than co-cultured macrophages or fresh blood monocytes. DCs displayed typical cytoplasmic processes, limited nonspecific esterase activity, and acquired antigen by phagocytosis, pinocytosis, and binding to specific receptors. Cytokine-exposed cells induced proliferation of allogeneic lymphocytes. Thus, the cells derived by these culture conditions had markers and functions analogous to immature myeloid DCs. Availability of feline DCs will enable investigation of their role in infectious disease and their potential therapeutic application. (C) 2003 Elsevier B.V. All rights reserved.