Serum Free Thiols Are Superior to Fecal Calprotectin in Reflecting Endoscopic Disease Activity in Inflammatory Bowel Disease
Serum Free Thiols Are Superior to Fecal Calprotectin in Reflecting Endoscopic Disease Activity in Inflammatory Bowel Disease
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DOI：
10.3390/antiox8090351
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发表时间：
2019-09-01
期刊：
影响因子：
7
通讯作者：
Dijkstra, Gerard
中科院分区：
文献类型：
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作者：
Bourgonje, Arno R.;Gabriels, Ruben Y.;Dijkstra, Gerard
氧化应激在炎症性肠病(IBD)的发病机制中起着关键作用。血清游离硫醇(R-SH)是反映全身氧化应激的可靠指标，因为它们很容易被活性物质氧化。在这里，我们的目标是建立IBD患者血清游离硫醇的浓度，并评估其对内窥镜疾病活动的区分能力。对78例IBD患者(31例克罗恩病(CD)和47例溃疡性结肠炎(UC))和50名健康对照进行了白蛋白调整后血清游离硫醇浓度的测定，并分析了与疾病参数的相关性及其对有相关数据的患者的内窥镜疾病活动性(n=54)或粪便钙保护蛋白(n=36)的判别价值。CD组和UC组血清游离硫醇浓度分别为19.4+/-3.1mU/g和17.8+/-3.4mU/g，显著低于健康对照组21.1+/-1.9mU/g白蛋白(P<；0.001)。游离硫醇高度准确地区分轻度和中到重度疾病活动，优于粪便钙保护素(FC)水平(分别为AUC0.87，P；0.001和AUC0.76，P；0.001)，并在交叉验证后保持不变。与健康对照组相比，IBD患者血清游离硫醇水平降低，且与内窥镜疾病活动程度密切相关。量化IBD的全身性氧化还原状态可能是监测IBD疾病活动性的一种有前途的微创方法。
Oxidative stress plays a pivotal role in the pathogenesis of inflammatory bowel diseases (IBD). Serum free thiols (R-SH) reliably reflect systemic oxidative stress, since they are readily oxidized by reactive species. Here, we aimed to establish concentrations of serum free thiols in IBD and assessed their discriminating capacity regarding endoscopic disease activity. Albumin-adjusted serum free thiol concentrations were measured in 78 IBD patients (31 Crohn's disease (CD) and 47 ulcerative colitis (UC) patients) and 50 healthy controls and analyzed for associations with disease parameters and their discriminative value regarding endoscopic disease activity (n = 54) or fecal calprotectin (n = 36) in patients for which those data were available. Mean serum free thiol concentrations were significantly lower in both CD and UC as compared to healthy controls (19.4 +/- 3.1 and 17.8 +/- 3.4 vs. 21.1 +/- 1.9 mu mol/g albumin, P < 0.001). Free thiols highly accurately discriminated between mild and moderate-to-severe disease activity, better than fecal calprotectin (FC) levels (AUC = 0.87, P < 0.001 vs. AUC = 0.76, P < 0.05, respectively) and this was maintained after cross-validation (AUC = 0.89, P < 0.001). Serum free thiols are reduced in IBD as compared to healthy controls and strongly correlate with the degree of endoscopic disease activity. Quantifying systemic redox status in IBD may be a promising, minimally invasive strategy to monitor IBD disease activity.