New comparative genomics approach reveals a conserved health span signature across species
New comparative genomics approach reveals a conserved health span signature across species
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DOI：
10.18632/aging.100342
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发表时间：
2011-06-01
期刊：
影响因子：
5.2
通讯作者：
Neretti, Nicola
中科院分区：
文献类型：
--
作者：
Antosh, Michael;Fox, David;Neretti, Nicola
环境和遗传干预通过引发不同特定但互补的途径的变化，延长了一系列生物体的健康寿命。我们研究了通过不同的干预措施延长健康跨度时不同物种间基因表达的变化。为了使用来自不同测量平台和生物体的异构数据集进行比较，我们开发了一种新的非参数方法，可以检测基因排名列表中重叠的统计显著性，并估计具有共同表达谱的基因数量。通过比较导致无脊椎动物和脊椎动物健康跨度持续增加的遗传和环境干预，我们建立了一个保守的健康跨度特征，并描述了这种特征如何依赖于组织类型。此外，我们研究了热量限制和白藜芦醇的关系，并首次在无脊椎动物和哺乳动物中发现了热量限制和白藜芦醇的共同基因和途径变化。因此，我们的方法可以用来探索和更好地定义高度复杂的生物现象之间的关系，在这种情况下，那些影响健康和寿命的现象。
Environmental and genetic interventions extend health span in a range of organisms by triggering changes in different specific but complementary pathways. We investigated the gene expression changes that occur across species when health span is extended via different interventions. To perform this comparison using heterogeneous datasets from different measurement platforms and organisms, we developed a novel non-parametric methodology that can detect statistical significance of overlaps in ranked lists of genes, and estimate the number of genes with a common expression profile. By comparing genetic and environmental interventions that consistently lead to increased health span in invertebrates and vertebrates we built a conserved health span signature and described how such a signature depends on tissue type. Furthermore, we examined the relationship between calorie restriction and resveratrol administration and for the first time, identified common gene and pathway changes in calorie restriction and resveratrol in both invertebrates and mammals. Our approach can thus be used to explore and better define the relationships between highly complex biological phenomena, in this case those that affect the health and longevity.