[2‐3H]GLYCEROL AS A PRECURSOR OF PHOSPHOLIPIDS IN RAT BRAIN: EVIDENCE FOR LACK OF RECYCLING
[2‐3H]GLYCEROL AS A PRECURSOR OF PHOSPHOLIPIDS IN RAT BRAIN: EVIDENCE FOR LACK OF RECYCLING
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[2-3H]甘油作为大鼠脑中磷脂的前体：缺乏回收的证据
DOI：
10.1111/j.1471-4159.1973.tb00081.x
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发表时间：
1973
影响因子：
4.7
通讯作者：
G. Mckhann
中科院分区：
文献类型：
--
作者：
J. Benjamins;G. Mckhann
摘要：当将[2‐3H]甘油注入幼龄大鼠脑内时，它以脉冲标签的形式呈现，迅速离开大脑，并将其标记的大部分氢释放为水。[2‐3H]甘油被有效地掺入脑脂质，尤其是胆碱和乙醇胺磷脂。注射[3H]‐和[14C]‐标记的甘油混合物后，全脑和微粒体部分磷脂中3H与14C的比例随注射后时间的变化而下降。这一发现表明氚标签的再循环较少。发现94%的磷脂酰胆碱的氚标签在分子的甘油部分而不是脂肪酸部分，进一步表明了这种缺乏回收。注射[3H]甘油2周后，脑内总放射性的93%出现在脂质部分。相比之下，注射[14C]甘油后，只有57%的放射性出现在脂质中，大约20%出现在蛋白质中。
Abstract— When [2‐3H]glycerol was injected intracranially into young rats, it was presented as a pulse label, leaving the brain rapidly and giving up much of its labelled hydrogen to water. [2‐3H]glycerol was efficiently incorporated into brain lipids, especially into choline and ethanolamine phospholipids. Following injection of a mixture of [3H]‐ and [14C]‐labelled glycerol, the ratio of 3H to 14C in the phospholipids of both whole brain and the microsomal fraction decreased as a function of time after injection. This finding indicated less recycling of the tritium label. This lack of recycling was further indicated by the finding that 94 per cent of the tritium label of phosphatidyl choline was in the glycerol portion of the molecule rather than in the fatty acids. At 2 weeks following injection with [3H]glycerol, 93 per cent of the total radioactivity in brain appeared in the lipid fraction. In contrast, following injection with [14C]glycerol, only 57 per cent of the radioactivity appeared in lipid, with about 20 per cent in protein.