Regulated expression of osteopontin in the peri-implantation rabbit uterus
Regulated expression of osteopontin in the peri-implantation rabbit uterus
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DOI：
10.1095/biolreprod.101.001347
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发表时间：
2003-05-01
影响因子：
3.6
通讯作者：
Lessey, BA
中科院分区：
文献类型：
--
作者：
Apparao, KBC;Illera, MJ;Lessey, BA
在早期着床过程中，胚泡附着在哺乳动物子宫内膜上涉及滋养层与子宫上皮表面的初始并置。骨桥蛋白（OPN）是哺乳动物子宫腺上皮在着床时分泌的细胞外基质的糖蛋白成分。这种蛋白质被整合素家族的几个成员识别，并促进细胞-细胞附着和粘附。本研究应用北方杂交和原位杂交技术检测了兔子宫OPN mRNA在妊娠早期的时空分布。北方印迹分析表明，在妊娠第4-7天，OPN表达显著增加，对应于该物种中循环孕酮的升高和初始胚胎附着的时间。原位杂交分析显示OPN mRNA在妊娠第6.75天表达，在子宫内膜上皮中表达最明显。使用免疫荧光法，OPN蛋白存在于妊娠第6.75天的腺上皮上，但在囊胚上不存在。此外，在非妊娠子宫内膜中未发现OPN mRNA或蛋白的表达。在单用孕酮处理的未交配兔中观察到子宫内膜OPN表达的诱导，并通过与抗孕酮ZK137.316共同处理来预防。雌激素-17 β本身对骨桥蛋白的表达没有影响，雌激素预激不需要证明孕酮的刺激作用。在兔子子宫中，与研究的其他哺乳动物物种一样，OPN在围着床期由子宫内膜腺体以阶段特异性方式表达，并受黄体酮调节。
Blastocyst attachment to the lining of the mammalian uterus during early implantation involves the initial apposition of the trophoblast to the uterine epithelial surface. Osteopontin (OPN) is a glycoprotein component of the extracellular matrix that is secreted by the glandular epithelium of mammalian uteri at the time of implantation. This protein is recognized by several members of the integrin family and promotes cell-cell attachment and adhesion. In the present study, rabbit uteri were examined using Northern and in situ hybridization to evaluate the temporal and spatial distribution of OPN mRNA during early pregnancy. Northern blot analysis demonstrated a dramatic increase in OPN expression on Days 4-7 of pregnancy, corresponding to the rise in circulating progesterone and the time of initial embryo attachment in this species. in situ hybridization analysis revealed OPN mRNA expression on Day 6.75 of pregnancy, which was most prominent on endometrial epithelium. Using immunofluorescence, OPN protein was present on the glandular epithelium on Day 6.75 of pregnancy, but was absent on blastocysts. Further, no expression of OPN mRNA or protein was found in the nonpregnant endometrium. Induction of endometrial OPN expression was observed in unmated rabbits treated with progesterone alone and was prevented by cotreatment with the antiprogestin ZK137.316. Estradiol-17beta had no effect on OPN expression by itself, and estrogen priming was not necessary to demonstrate the stimulatory effect of progesterone. In The rabbit uterus, as in other mammalian species studied, OPN is expressed in a stage-specific manner by the endometrial glands during the peri-implantation period and is regulated by progesterone.