Exopolysaccharides produced by the recently described halophilic bacteria Halomonas ventosae and Halomonas anticariensis
Exopolysaccharides produced by the recently described halophilic bacteria Halomonas ventosae and Halomonas anticariensis
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DOI：
10.1016/j.resmic.2006.06.004
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发表时间：
2006-11-01
影响因子：
2.6
通讯作者：
Quesada, Emilia
中科院分区：
文献类型：
--
作者：
Antonio Mata, Juan;Bejar, Victoria;Quesada, Emilia
本文研究了两种新的嗜盐菌-芬托盐单胞菌（Halomonaspentosae）和安替卡盐单胞菌（Halomonaspanticariensis）产生的胞外多糖（EPS）。在适宜的环境和营养条件下，H. ventosae菌株A112（T）和A116每100 ml培养基分别分泌28.35 mg和28.95 mg EPS（每克细胞干重分别分泌34.55和38.6 mg EPS）。聚合物的分子量约为50 kDa，其主要成分为葡萄糖、甘露糖和半乳糖。它们具有高蛋白组分，并在几种疏水底物上表现出乳化活性。在适宜的环境和营养条件下，H. anticariensis菌株FP 35（T）和FP 36每100 ml培养基分别分泌约29.65和49.95 mg EPS（每克干细胞重43.6和50.95 mg EPS）。聚合物的分子量分别约为20和46 kDa，主要由葡萄糖、甘露糖和半乳糖醛酸组成。所有的EPS产生的解决方案的低粘度和假塑性行为。它们还具有结合阳离子的高能力，并结合了大量的硫酸盐，这在细菌多糖中是非常不寻常的。所有测定的菌株在聚苯乙烯威尔斯孔和硼硅酸盐试管中均形成生物膜。(c)2006年，Elsevier Masson SAS。All rights reserved.
We studied exopolysaccharides (EPSs) produced by Halomonas ventosae and Halomonas anticariensis, two novel species of halophilic bacteria. Under optimum environmental and nutritional conditions, H. ventosae strains A112(T) and A116 excreted 28.35 mg and 28.95 mg of EPS per 100 ml of culture medium (34.55 and 38.6 mg of EPS per gram of dry cell weight) respectively. The molecular masses of the polymers were about 50 kDa and their main components were glucose, mannose and galactose. They had high protein fractions and showed emulsifying activity on several hydrophobic substrates. Under optimum environmental and nutritional conditions, H. anticariensis strains FP35(T) and FP36 excreted about 29.65 and 49.95 mg of EPS per 100 ml of culture medium (43.6 and 50.95 mg of EPS per gram of dry cell weight) respectively. The molecular masses of the polymers were about 20 and 46 kDa respectively and were composed mainly of glucose, mannose and galacturonic acid. All EPSs produced solutions of low viscosity and pseudoplastic behaviour. They also had a high capacity for binding cations and incorporated considerable quantities of sulphates, which is highly unusual in bacterial polysaccharides. All strains assayed formed biofilms both in polystyrene wells and borosilicate test tubes. (c) 2006 Elsevier Masson SAS. All rights reserved.