Optical coherence micro-elastography: mechanical-contrast imaging of tissue microstructure
Optical coherence micro-elastography: mechanical-contrast imaging of tissue microstructure
复制标题
DOI：
10.1364/boe.5.002113
复制
发表时间：
2014-07-01
影响因子：
3.4
通讯作者：
Sampson, David D.
中科院分区：
文献类型：
--
作者：
Kennedy, Brendan F.;McLaughlin, Robert A.;Sampson, David D.
我们提出了光学相干微弹性成像，这是压缩光学相干弹性成像的改进形式。我们展示了这种技术产生正面图像的能力，与组织学密切相关，揭示了人类乳房和淋巴结组织的微观机械对比。我们使用相敏的三维光学相干断层扫描（OCT）来探测压缩载荷引起的组织中纳米到微米尺度的轴向位移。光学相干微弹性成像结合了共路干涉测量、复杂OCT信号的加权平均和加权最小二乘回归。使用三维相位展开，我们将最大可检测应变增加了11倍，最小可检测应变为2.6 mu epsilon。我们展示了机械对比在人类乳腺癌病理和淋巴结形态中可视化微观组织结构的潜力。(C) 2014年美国光学学会
We present optical coherence micro-elastography, an improved form of compression optical coherence elastography. We demonstrate the capacity of this technique to produce en face images, closely corresponding with histology, that reveal micro-scale mechanical contrast in human breast and lymph node tissues. We use phase-sensitive, three-dimensional optical coherence tomography (OCT) to probe the nanometer-to-micrometer-scale axial displacements in tissues induced by compressive loading. Optical coherence micro-elastography incorporates common-path interferometry, weighted averaging of the complex OCT signal and weighted least-squares regression. Using three-dimensional phase unwrapping, we have increased the maximum detectable strain eleven-fold over no unwrapping and the minimum detectable strain is 2.6 mu epsilon. We demonstrate the potential of mechanical over optical contrast for visualizing micro-scale tissue structures in human breast cancer pathology and lymph node morphology. (C) 2014 Optical Society of America