Research Resource: Comprehensive Expression Atlas of the Fibroblast Growth Factor System in Adult Mouse
Research Resource: Comprehensive Expression Atlas of the Fibroblast Growth Factor System in Adult Mouse
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DOI：
10.1210/me.2010-0142
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发表时间：
2010-10-01
影响因子：
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通讯作者：
Kliewer, Steven A.
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文献类型：
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作者：
Tacer, Klementina Fon;Bookout, Angie L.;Kliewer, Steven A.
尽管成纤维细胞生长因子 (FGF) 家族的成员及其受体在胚胎发生中具有明确的作用，但它们对成人生理学的贡献仍然相对未经探索。在这里，我们使用实时定量 PCR 来确定 39 种不同小鼠组织中所有 22 种 FGF、七种主要 FGF 受体 (FGFR) 以及 Klotho 辅助受体家族的三个成员的 mRNA 表达模式。对 mRNA 表达数据的无监督层次聚类分析表明，大多数 FGF 和 FGFR 分为两组，其表达在中枢神经系统或生殖和胃肠组织中富集。有趣的是，可以充当内分泌激素的 FGF，包括 FGF15/19、FGF21 和 FGF23，聚集在不包括任何 FGFR 的第三组中，强调了它们在组织间信号传导中的作用。我们进一步表明，最近鉴定的 Klotho 家族成员乳糖酶样在棕色脂肪组织和眼睛中高度选择性表达，并且可以作为 FGF19 的附加辅助受体。该 FGF 图谱为指导未来研究阐明 FGF 在成年动物中的生理功能提供了重要资源。 （分子内分泌学24：2050-2064，2010）
Although members of the fibroblast growth factor (FGF) family and their receptors have well-established roles in embryogenesis, their contributions to adult physiology remain relatively unexplored. Here, we use real-time quantitative PCR to determine the mRNA expression patterns of all 22 FGFs, the seven principal FGF receptors (FGFRs), and the three members of the Klotho family of coreceptors in 39 different mouse tissues. Unsupervised hierarchical cluster analysis of the mRNA expression data reveals that most FGFs and FGFRs fall into two groups the expression of which is enriched in either the central nervous system or reproductive and gastrointestinal tissues. Interestingly, the FGFs that can act as endocrine hormones, including FGF15/19, FGF21, and FGF23, cluster in a third group that does not include any FGFRs, underscoring their roles in signaling between tissues. We further show that the most recently identified Klotho family member, Lactase-like, is highly and selectively expressed in brown adipose tissue and eye and can function as an additional coreceptor for FGF19. This FGF atlas provides an important resource for guiding future studies to elucidate the physiological functions of FGFs in adult animals. (Molecular Endocrinology 24: 2050-2064, 2010)