Assembly of large genomes using second-generation sequencing
Assembly of large genomes using second-generation sequencing
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DOI：
10.1101/gr.101360.109
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发表时间：
2010-09-01
期刊：
影响因子：
7
通讯作者：
Salzberg, Steven L.
中科院分区：
文献类型：
--
作者：
Schatz, Michael C.;Delcher, Arthur L.;Salzberg, Steven L.
现在，第二代测序技术可在几天内以低成本对整个人类基因组进行测序。序列读长最初非常短，自该技术首次出现以来已迅速增加，我们现在看到越来越多的努力从这些短读长中对大型基因组进行测序。在本视角中，我们描述了与短读组装相关的问题、第二代测序仪产生的不同类型的数据以及为这些数据设计的最新组装算法。我们还审查了最近通过短读段组装的基因组，并就产生高质量组装的测序策略提出了建议。
Second-generation sequencing technology can now be used to sequence an entire human genome in a matter of days and at low cost. Sequence read lengths, initially very short, have rapidly increased since the technology first appeared, and we now are seeing a growing number of efforts to sequence large genomes de novo from these short reads. In this Perspective, we describe the issues associated with short-read assembly, the different types of data produced by second-gen sequencers, and the latest assembly algorithms designed for these data. We also review the genomes that have been assembled recently from short reads and make recommendations for sequencing strategies that will yield a high-quality assembly.