GIGANTEA is a co-chaperone which facilitates maturation of ZEITLUPE in the Arabidopsis circadian clock.
GIGANTEA is a co-chaperone which facilitates maturation of ZEITLUPE in the Arabidopsis circadian clock.
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DOI：
10.1038/s41467-016-0014-9
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发表时间：
2017-02-23
影响因子：
16.6
通讯作者：
Somers DE
中科院分区：
文献类型：
--
作者：
Cha JY;Kim J;Kim TS;Zeng Q;Wang L;Lee SY;Kim WY;Somers DE
昼夜节律时钟系统有助于建立适当协调生理和新陈代谢所需的行为、发育和分子事件的正确日常阶段。昼夜节律振荡器包含转录-翻译反馈环路，但也需要调节时钟蛋白稳态的翻译后过程。 GIGANTEA 是一种独特的植物蛋白，参与维持和控制植物生理和发育的众多方面。 GIGANTEA 通过一种未知的机制稳定 F-box 蛋白 ZEITLUPE，这是生物钟的关键调节因子。在这里，我们证明 GIGANTEA 具有一般的蛋白质伴侣活性，并且可以特异性地促进 ZEITLUPE 在体外和植物中成熟为活性形式。 GIGANTEA 与 HSP90 和 ZEITLUPE 形成三元复合物，其共伴侣作用可协同增强 ZEITLUPE 的 HSP90/HSP70 体外成熟。这些结果确定了 GIGANTEA 活性的分子机制，可以解释其在植物生物学中的广泛作用。植物特有的 GIGANTEA 蛋白通过一种未知的机制稳定 F-box 蛋白 ZEITLUPE 来调节生物钟。这里查等人。表明 GIGANTEA 具有内在的伴侣活性，并且可以通过与 HSP90 协同作用促进 ZEITLUPE 成熟。
Circadian clock systems help establish the correct daily phasing of the behavioral, developmental, and molecular events needed for the proper coordination of physiology and metabolism. The circadian oscillator comprises transcription–translation feedback loops but also requires post-translational processes that regulate clock protein homeostasis. GIGANTEA is a unique plant protein involved in the maintenance and control of numerous facets of plant physiology and development. Through an unknown mechanism GIGANTEA stabilizes the F-box protein ZEITLUPE, a key regulator of the circadian clock. Here, we show that GIGANTEA has general protein chaperone activity and can act to specifically facilitate ZEITLUPE maturation into an active form in vitro and in planta. GIGANTEA forms a ternary complex with HSP90 and ZEITLUPE and its co-chaperone action synergistically enhances HSP90/HSP70 maturation of ZEITLUPE in vitro. These results identify a molecular mechanism for GIGANTEA activity that can explain its wide-ranging role in plant biology. The plant-specific GIGANTEA protein regulates the circadian clock by stabilizing the F-box protein ZEITLUPE via an unknown mechanism. Here Cha et al. show that GIGANTEA has intrinsic chaperone activity and can facilitate ZEITLUPE maturation by acting synergistically with HSP90.