Co-generation of bio-butanol and bio-lipids under a hybrid process
Co-generation of bio-butanol and bio-lipids under a hybrid process
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混合工艺联产生物丁醇和生物脂质
DOI：
10.1039/c5gc02285a
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发表时间：
2016-01-01
期刊：
影响因子：
9.8
通讯作者：
Tan, Tianwei
中科院分区：
文献类型：
--
作者：
DiCai;Dong, Zhongshi;Tan, Tianwei
本研究建立了一种新型的生物丁醇和油脂的混合发酵工艺。将从两阶段发酵获得的含有ABE的发酵液用作分批生物脂质发酵的底物。气提单元与生物脂质生物反应器集成以分离ABE产物。载气还为产油微生物的代谢提供无菌空气。考察了接种量对生物油脂发酵的影响，并进行了优化。在优化的条件下，使用合成培养基（10%的接种率和额外的碳源补充），ABE发酵液中约87.9%的初始COD被降解，而类似于6.4 g L-1的生物脂质和类似于82.1 g L-1的ABE溶剂分别累积在生物脂质生物反应器和气提单元的冷凝物中。此外，ABE发酵液中的有机酸副产物可以作为碳源重复使用，用于红酵母属微生物油脂生产，这是以前没有报道过的。以甜高粱渣酶解液为底物，进一步研究了该复合工艺。当直接使用水解产物而不解毒时，类似于62.2 g L-1的ABE溶剂和类似于2.7 g L-1的生物脂质同时产生，COD降解率类似于70.1%。相应地，0.06 g生物脂质与0.25 g ABE产物从1 g还原糖在木质纤维素水解产物中的混合物共同产生。混合生物炼制工艺为生物燃料的生产提供了一种节能的方式，具有环境效益。
A novel hybrid fermentation process for the production of biological butanol and lipids was established in this study. The ABE containing broth obtained from the two-stage fermentation was used as the substrate for the batch bio-lipids fermentation. A gas stripping unit was integrated with the bio-lipids bioreactor to separate the ABE products. The carrier gas also provided the sterilized air for the metabolism of oleaginous microorganisms. The effect of inoculation rate on the bio-lipids fermentation was evaluated and optimized. Under the optimized conditions using a synthetic medium (10% of inoculation rate with additional carbon source supplementation), about 87.9% of the initial COD in the ABE fermentation broth was degraded, while similar to 6.4 g L-1 of the bio-lipids and similar to 82.1 g L-1 of the ABE solvent cumulated in the bio-lipids bioreactor and the condensate of the gas stripping unit, respectively. Moreover, the organic acid by-products in the ABE broth could be reused as the carbon source for microbial lipid production using Rhodotorula sp., which was not reported before. The hybrid process was further performed using enzymatic hydrolysate of sweet sorghum bagasse as the substrate. When using the hydrolysate directly without detoxification, similar to 62.2 g L-1 of the ABE solvents and similar to 2.7 g L-1 of the bio-lipids were produced simultaneously with a COD degradation rate of similar to 70.1% from the ABE broth. Correspondingly, 0.06 g of the bio-lipids was co-generated with 0.25 g of the ABE products from a mixture of 1 g reducing sugar in lignocellulosic hydrolysate. The hybrid biorefinery process provides an energy saving way for the manufacture of bio-fuels with environmental benefits.