The BIR and BIR-like domains of Bombyx mori nucleopolyhedrovirus IAP2 protein are required for efficient viral propagation
The BIR and BIR-like domains of Bombyx mori nucleopolyhedrovirus IAP2 protein are required for efficient viral propagation
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DOI：
10.1016/j.bbrc.2014.10.132
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发表时间：
2014-11-28
影响因子：
3.1
通讯作者：
Katsuma, Susumu
中科院分区：
文献类型：
--
作者：
Ito, Hanako;Bando, Hisanori;Katsuma, Susumu
杆状病毒核型多角体病毒（BmNPV）具有两个基因iap1和iap2，它们编码凋亡抑制蛋白（IAP）。我们以前表明，虽然这两个基因都是病毒繁殖所必需的，iap2是培养细胞中有效的病毒繁殖所必需的。BmNPV IAP 2含有3个功能结构域：杆状病毒IAP重复序列（BIR）、BIR样结构域（BIRL）和RING指结构域。为了鉴定影响病毒生长的结构域，我们产生了一系列表达iap2衍生物的BmNPV杆状病毒，所述iap2衍生物缺少一个或两个结构域，或具有单个氨基酸取代以消除IAP2泛素连接酶活性。我们在培养细胞和B中检测了它们的性质。mod幼虫我们发现IAP 2的BIR或BIRL结构域在BmNPV感染中起着重要作用，而RING指结构域，这是泛素连接酶活性所需的，并没有很大的贡献BmNPV的繁殖。这是第一个研究，以确定杆状病毒IAP 2蛋白的功能结构域。(C)2014爱思唯尔公司All rights reserved.
The baculovirus Bombyx mod nucleopolyhedrovirus (BmNPV) possesses two genes, iap1 and iap2, which encode inhibitor of apoptosis (IAP) proteins. We previously showed that although both genes are dispensable for viral propagation, iap2 is required for efficient viral propagation in cultured cells. BmNPV IAP2 contains three putative functional domains: a baculovirus IAP repeat (BIR), a BIR-like (BIRL) domain, and a RING finger domain. To identify the domain affecting viral growth, we generated a series of BmNPV bacmids expressing iap2 derivatives lacking one or two domains, or possessing a single amino acid substitution to abolish IAP2 ubiquitin ligase activity. We examined their properties in both cultured cells and B. mod larvae. We found that either the BIR or BIRL domain of IAP2 plays an important role in BmNPV infection, and that the RING finger domain, which is required for ubiquitin ligase activity, does not greatly contribute to BmNPV propagation. This is the first study to identify functional domains of the baculovirus IAP2 protein. (C) 2014 Elsevier Inc. All rights reserved.