MiR-582-5p Inhibits Bladder Cancer-Genesis by Suppressing TTK Expression.
MiR-582-5p Inhibits Bladder Cancer-Genesis by Suppressing TTK Expression.
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DOI：
10.2147/cmar.s274835
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发表时间：
2020
影响因子：
3.3
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Tian Y;Guan Y;Su Y;Luo W;Yang G;Zhang Y
膀胱癌（BC）是指在膀胱的细胞和组织中发现的恶性生长。虽然许多研究已经研究了BC的进展，但科学家们尚未完全了解BC的机制。本研究旨在探讨miR-582 - 5p及其靶基因TTK在乳腺癌发病机制中的作用。使用qRT-PCR评估BC组织或细胞中的miR-582 - 5p和TTK mRNA表达。然后使用TargetScan预测miR-582 - 5p在TTK mRNA上的结合位点。随后，采用双荧光素酶报告基因和RNA下拉测定来验证miR-582 - 5p与TTK mRNA之间的结合关系。随后进行CCK-8、BrdU、流式细胞术和caspase-3活性测定以评估BC细胞的活力、增殖、细胞周期和凋亡。研究发现miR-582 - 5p在BC组织和细胞中下调。同时，miR-582 - 5p可抑制BC细胞的活力和增殖，促进细胞凋亡和周期阻滞。后来发现miR-582 - 5p的靶基因TTK在BC组织和细胞中过表达。然而，观察到TTK对miR-582 - 5p表现出相反的作用。简单地说，它刺激BC细胞恶性表型，并且这种刺激可以被miR-582 - 5p直接逆转。本研究证实miR-582 - 5p可通过抑制TTK表达而抑制膀胱癌的发生。
Bladder cancer (BC) refers to the malignant growth found in the cells and tissues of the urinary bladder. While many studies have researched the progression of BC, scientists are yet to fully understand the mechanism of BC. This research aimed to explore the role of miR-582-5p and its target gene TTK in BC pathogenesis. The evaluation of miR-582-5p and TTK mRNA expression in BC tissues or cells was performed using qRT-PCR. TargetScan was then used to predict the binding site of miR-582-5p on TTK mRNA. Subsequently, dual-luciferase reporter and RNA pull-down assays were employed to validate the binding relationship between miR-582-5p and TTK mRNA. CCK-8, BrdU, flow cytometry, and caspase-3 activity assays were later conducted to evaluate the viability, proliferation, cell cycle, and apoptosis of BC cells. Investigations revealed that miR-582-5p was downregulated in BC tissues and cells. Meanwhile, miR-582-5p inhibited the viability and proliferation of BC cells while stimulating the apoptosis and cycle arrest of the cells. TTK, the target gene of miR-582-5p, was later found to be over-expressed in BC tissues and cells. TTK, however, was observed to exhibit an opposite effect on miR-582-5p. Simply put, it stimulated BC cell malignant phenotypes, and this stimulation could be directly reversed by miR-582-5p. This research confirmed that miR-582-5p could restrain bladder carcinogenesis by inhibiting TTK expression.