The LC7 light chains of Chlamydomonas flagellar dyneins interact with components required for both motor assembly and regulation
The LC7 light chains of Chlamydomonas flagellar dyneins interact with components required for both motor assembly and regulation
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DOI：
10.1091/mbc.e04-06-0461
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发表时间：
2004-10-01
影响因子：
3.3
通讯作者：
King, SM
中科院分区：
文献类型：
--
作者：
DiBella, LM;Sakato, M;King, SM
轻链（LC）的LC 7/路障家族的成员已经在细胞质和轴丝动力蛋白中发现。LC 7a最初在衣原体外臂动力蛋白中被鉴定，并与该马达的货物结合区相关。我们在这里描述了一个新的成员，这个蛋白质家族，称为LC 7 B，也存在于衣原体鞭毛。在从缺少内臂11的菌株分离的轴丝中，LC 7 b的水平降低了约20%，在缺少外臂的菌株中，LC 7 b的水平降低了约80%。当两种动力蛋白缺失时，LC 7 b水平降低至
Members of the LC7/Roadblock family of light chains (LCs) have been found in both cytoplasmic and axonemal dyneins. LC7a was originally identified within Chlamydomonas outer arm dynein and associates with this motor's cargo-binding region. We describe here a novel member of this protein family, termed LC7b that is also present in the Chlamydomonas flagellum. Levels of LC7b are reduced similar to20% in axonemes isolated from strains lacking inner arm 11 and are similar to80% lower in the absence of the outer arms. When both dyneins are missing, LC7b levels are diminished to