Electronic control of gene expression and cell behaviour in Escherichia coli through redox signalling.
Electronic control of gene expression and cell behaviour in Escherichia coli through redox signalling.
复制标题
通过氧化还原信号传导，大肠杆菌中基因表达和细胞行为的电子控制。
DOI：
10.1038/ncomms14030
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发表时间：
2017-01-17
影响因子：
16.6
通讯作者：
Bentley WE
中科院分区：
文献类型：
--
作者：
Tschirhart T;Kim E;McKay R;Ueda H;Wu HC;Pottash AE;Zargar A;Negrete A;Shiloach J;Payne GF;Bentley WE
在电子和离子形式之间相互转换信息的能力已经改变了我们记录和启动生物功能的能力。合成生物学通过使用生物分子和提供对基于氧化还原的细胞信号和行为的电化学访问，提供了扩大通信“带宽”的潜力。虽然工程细胞已经将分子信息传输到电子设备，但双向通信的潜力在很大程度上尚未开发。在这里，我们提出了一个简单的电致装置，使用氧化还原生物分子携带电子信息的工程细菌细胞，以控制从一个简单的合成基因电路的转录。天然转录调节因子SoxR的电子致动和来自PsoxS启动子的转录允许快速、可逆且依赖于所施加的电子信号的振幅和频率的细胞应答。此外，细菌运动性和基于群体的细胞间通讯的诱导证明了我们方法的多功能性和驱动复杂生物行为的潜力。合成生物学提供了探索操纵基因表达和功能的新方法的能力。在这里，作者展示了一种电遗传装置，允许通过外源电信号控制转录。
The ability to interconvert information between electronic and ionic modalities has transformed our ability to record and actuate biological function. Synthetic biology offers the potential to expand communication ‘bandwidth' by using biomolecules and providing electrochemical access to redox-based cell signals and behaviours. While engineered cells have transmitted molecular information to electronic devices, the potential for bidirectional communication stands largely untapped. Here we present a simple electrogenetic device that uses redox biomolecules to carry electronic information to engineered bacterial cells in order to control transcription from a simple synthetic gene circuit. Electronic actuation of the native transcriptional regulator SoxR and transcription from the PsoxS promoter allows cell response that is quick, reversible and dependent on the amplitude and frequency of the imposed electronic signals. Further, induction of bacterial motility and population based cell-to-cell communication demonstrates the versatility of our approach and potential to drive intricate biological behaviours. Synthetic biology offers the ability to explore new ways of manipulating gene expression and function. Here the authors demonstrate an electrogenetic device that allows control of transcription by an exogenous electrical signal.