Near-infrared light-triggered drug release from a multiple lipid carrier complex using an all-in-one strategy
Near-infrared light-triggered drug release from a multiple lipid carrier complex using an all-in-one strategy
复制标题
采用一体化策略，近红外光触发多种脂质载体复合物释放药物。
DOI：
10.1016/j.jconrel.2017.06.029
复制
发表时间：
2017-09-10
影响因子：
10.8
通讯作者：
Sun, Minjie
中科院分区：
文献类型：
--
作者：
Li, Huipeng;Yang, Xue;Sun, Minjie
本研究报道了一种由脂质体内的纳米结构脂质载体（NLCs）组成的药物递送系统（Lip - NLCs）。这种多层脂质载体复合物具有激光触发的响应性药物释放特性。通过采用一体化策略，疏水性和亲水性药物均可载入同一制剂中。我们假设，若将疏水性近红外（NIR）染料IR780载入脂质体磷脂双分子层，激光照射会破坏该双分子层，从而在肿瘤部位远程触发药物释放。我们运用体外和体内实验方法证实，激光照射有助于疏水性和亲水性药物的控释。近红外激光触发的药物释放程度可通过改变激光强度和照射时间进行调节。激光处理后，亲水性的AMD3100从脂质体水相腔室中释放出来，然后与肿瘤细胞表面的CXCR4受体结合，以抑制转移。携带亲脂性IR780的NLCs也从脂质体水相腔室中释放出来，并被肿瘤细胞摄取，从而增强IR780的光热治疗效果。更重要的是，载入IR780和AMD3100的Lip - NLCs（IR780 - AMD - Lip - NLCs）展现出增强的抗肿瘤和抗转移效果。这些结果表明，Lip - NLCs是一种安全且制备简单的一体化平台，可用于递送不同溶解性的药物。该系统便于对所载药物进行可控释放，以实现多功能联合治疗。
The present study reports a drug delivery system comprising nanostructured lipid carrier (NLCs) within liposomes (Lip-NLCs). This multiple lipid carrier complex features laser-triggered responsive drug release. Both hydrophobic and hydrophilic drugs can be loaded into the same formulation by applying an all-in-one strategy. We hypothesized that if we loaded the hydrophobic near-infrared (NIR) dye IR780 into the liposome phospholipid bilayer, the bilayer would be disrupted by laser irradiation so that drug release would be triggered remotely at the tumor site. We used in vitro and in vivo methods to verify that laser irradiation facilitated controlled release of both hydrophobic and hydrophilic drugs. The degree of drug release triggered by NIR laser light could be adjusted by varying the laser intensity and irradiation time. Following laser treatment, hydrophilic AMD3100 was released from the aqueous liposome chamber and then bound with CXCR4 receptors on the tumor cell surface to inhibit metastasis. NLCs carrying lipophilic IR780 were also released from the aqueous chamber of liposomes and taken up into tumor cells to enhance the photothermal therapeutic effect of IR780. More importantly, Lip-NLCs loaded with IR780 and AMD3100 (IR780-AMD-Lip-NLCs) exhibited enhanced anti-tumor and anti-metastasis effects. These results suggest that Lip-NLCs are a safe and simply prepared all-in-one platform for delivery of drugs with different solubilities. This system facilitates easily controlled release of cargoes to achieve multi-functional combined therapy.