blaNDM-1 Carriage on IncR Plasmid in Enterobacteriaceae Strains
blaNDM-1 Carriage on IncR Plasmid in Enterobacteriaceae Strains
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DOI：
10.1089/mdr.2015.0083
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发表时间：
2016-03-01
影响因子：
2.6
通讯作者：
Mazzariol, Annarita
中科院分区：
文献类型：
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作者：
Kocsis, Erika;Guzvinec, Marija;Mazzariol, Annarita
2009年至2011年，通过克罗地亚全国碳青霉烯类耐药肠杆菌科监测，分离出4株产生NDM-1的肠杆菌科菌株（3株肺炎克雷伯菌和1株克塞利Citrobacter koseri），研究bla（NDM）的分子遗传背景及其质粒类型。表型上，临床菌株被证明是多重耐药的。所有菌株对替加环素和粘菌素均敏感。临床菌株具有不同的抗生素耐药决定因素，特别是不同组合的bla（NDM-1）、bla（TEM-1）、bla（SHV-1）、bla（SHV-12）、bla（OXA-1）、bla（OXA-9）、bla（CTX-M-15、）bla（CMY-4、）qnrB1和aac（6′）Ib-cr。2株肺炎克雷伯菌属于ST15序列型，1株属于ST16序列型。在质粒类型上，柯氏梭菌和1株ST15肺炎克雷伯菌携带IncR， 2株ST15肺炎克雷伯菌携带IncR和colE。肺炎克雷伯菌ST16菌株携带A/C和colE质粒。在A/C型和IncR型共轭高分子量质粒上检测到bla（NDM-1）基因。值得注意的是，这是欧洲首次报道表达NDM-1的肺炎克雷伯菌ST16。值得注意的是，我们的研究强调了IncR质粒作为多药耐药储存库的重要性。据我们所知，携带bla（NDM-1）的IncR质粒在柯氏弧菌中为首次报道。
Four NDM-1-producing Enterobacteriaceae strains (three Klebsiella pneumoniae and one Citrobacter koseri) were isolated between 2009 and 2011 through a nationwide surveillance for carbapenem-resistant Enterobacteriaceae in Croatia to study the molecular genetic background of bla(NDM) and the responsible plasmid types. Phenotypically, the clinical strains proved to be multidrug resistant. All strains remained susceptible to tigecycline and colistin. The clinical strains harbored variable antibiotic resistance determinants, notably, bla(NDM-1,) bla(TEM-1), bla(SHV-1), bla(SHV-12), bla(OXA-1), bla(OXA-9), bla(CTX-M-15,) bla(CMY-4,) qnrB1, and aac(6 ')Ib-cr in different combinations. Two K. pneumoniae belonged to sequence type ST15 and one strain to ST16. As for the plasmid types, C. koseri and one of the ST15 K. pneumoniae carried IncR, and the second ST15 K. pneumoniae carried IncR and colE. The K. pneumoniae ST16 strain hosted A/C and colE plasmids. The bla(NDM-1) gene was detected on conjugative high-molecular-weight plasmids, namely, A/C and IncR types. It is noteworthy that this is the first description of K. pneumoniae ST16 expressing NDM-1 in Europe. Remarkably, our study underscores the importance of the IncR plasmid as a reservoir of multidrug resistance. To the best of our knowledge, the IncR plasmid carrying bla(NDM-1) in C. koseri is reported for the first time.