Differential protein profile in the ear-punched tissue of regeneration and non-regeneration strains of mice: a novel approach to explore the candidate genes for soft-tissue regeneration
Differential protein profile in the ear-punched tissue of regeneration and non-regeneration strains of mice: a novel approach to explore the candidate genes for soft-tissue regeneration
复制标题
DOI：
10.1016/s0304-4165(00)00118-5
复制
发表时间：
2000-12-15
影响因子：
3
通讯作者：
Baylink, DJ
中科院分区：
文献类型：
--
作者：
Li, X;Mohan, S;Baylink, DJ
伤口修复/再生是一个基因控制的复杂过程。为了鉴定调控软组织中快速伤口修复/再生的候选基因，使用表面增强激光解吸电离（SELDI）蛋白质芯片技术分析再生品系MRL/MpJ-Fas（lpr）（MRL）小鼠和非再生品系C57 BL/6 J（B6）小鼠的耳穿孔组织中软组织愈合过程的时间蛋白质谱。鉴定了五种候选蛋白，其中MRL对耳穿孔的反应与B6的反应相比有2-4倍的不同。使用抗原-抗体测定验证的基于质量的方法预测它们相应的基因。已知大多数预测基因在伤口修复/再生中起作用或可能起作用。在5种候选蛋白中，耳穿孔组织中23 560 Da蛋白的量与6种代表性品系小鼠的耳愈合率显著相关，使其成为快速伤口修复/再生的良好候选蛋白。我们推测伤口组织中23 560 Da蛋白浓度的增加可以刺激各种促生长蛋白的表达，从而加速伤口修复/再生过程。在这里，我们已经表明，使用SELDI技术，再加上数据库搜索的蛋白质表达谱的检查，是一种替代的方法来寻找候选基因的伤口修复/再生。这种新的方法可以在各种生物应用中实现。(C)2000 Elsevier Science B. V.保留所有权利。
Wound repair/regeneration is a genetically controlled, complex process. In order to identify candidate genes regulating fast wound repair/regeneration in soft-tissue, the temporal protein profile of the soft-tissue healing process was analyzed in the ear-punched tissue of regeneration strain MRL/MpJ-Fas(lpr) (MRL) mice and non-regeneration strain C57BL/6J(B6) mice using surface-enhanced laser desorption and ionization (SELDI) ProteinChip technology. Five candidate proteins were identified in which responses of MRL to the ear punch were 2-4-fold different compared to that of B6. Their corresponding genes were predicted using an antigen-antibody assay validated mass-based approach. Most of the predicted genes are known to play a role or are likely to play a role in the wound repair/regeneration Of the five candidate proteins, the amount of the 23 560 Da protein in the ear-punched tissue was significantly correlated with the rate of ear healing in six representative strains of mice, making it a good candidate for fast wound repair/regeneration. We speculate that the increased concentration of the 23 560 Da protein in the wound tissue could stimulate the expression of various growth-promoting proteins and consequently speed up the wound repair/regeneration processes. Here, we have shown that examination of protein expression profile using SELDI technology, coupled with database search, is an alternative approach to search for candidate genes for wound repair/regeneration. This novel approach can be implemented in a variety of biological applications. (C) 2000 Elsevier Science B.V. All rights reserved.