Prevention of TCR-mediated apoptosis by the elevation of cAMP.
Prevention of TCR-mediated apoptosis by the elevation of cAMP.
复制标题
通过提高 cAMP 来预防 TCR 介导的细胞凋亡。
DOI：
10.1093/intimm/6.7.1081
复制
发表时间：
1994
影响因子：
4.4
通讯作者：
Yoshihiro Asano
中科院分区：
文献类型：
--
作者：
Sumio Hoshi;Makoto Furutani;Masao Seto;Tomio Tada;Yoshihiro Asano
The stimulation through TCR-CD3 complexes by immobilized anti-CD3 antibody induced the production of IL-2 and activation-induced cell death (ACD) in the majority of T cell hybridomas. However, some hybridomas produced IL-2 without showing any signs of ACD by the same stimulation, indicating that TCR-CD3-mediated signaling pathways of IL-2 production and of ACD are different. These pathways were discriminated from each other by protein kinase inhibitors and cAMP-elevating reagents such as forskolin. The pathway of IL-2 production but not of ACD was inhibited by protein kinase inhibitors. On the other hand, various cAMP-elevating reagents prevented the T cell hybridomas from TCR-mediated ACD with minimal inhibition of IL-2 production. The elevated cytoplasmic cAMP did not block dexamethasone-induced apoptosis. This indicates that apoptosis is regulated by multiple pathways. Furthermore, the inhibitory effect of cAMP is specific for the TCR-mediated signaling pathway of ACD. Messenger RNA for bcl-2 was detected after treatment with forskolin.
