Influences of surface chemistry and swelling of salt-treated polyelectrolyte multilayers on migration of smooth muscle cells
Influences of surface chemistry and swelling of salt-treated polyelectrolyte multilayers on migration of smooth muscle cells
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盐处理聚电解质多层膜的表面化学和膨胀对平滑肌细胞迁移的影响
DOI：
10.1098/rsif.2012.0546
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发表时间：
2012-12-07
影响因子：
3.9
通讯作者：
Gao, Changyou
中科院分区：
文献类型：
--
作者：
Han, Lulu;Mao, Zhengwei;Gao, Changyou
细胞迁移在多种生理和病理过程中起着至关重要的作用，并且可以通过细胞-基质相互作用来调节。我们以前发现，聚（4-苯乙烯磺酸钠）（PSS）/聚（二烯丙基二甲基铵）氯化物（PDADMAC）多层膜后处理在1-5 M NaCl溶液中导致其物理化学性质的连续变化，如厚度，化学组成，表面电荷，溶胀比和润湿性。在这项研究中，人平滑肌细胞（SMC）对这些盐处理的多层膜的反应，特别是细胞迁移的控制因素，为设计治疗和植入物提供了原则，被公开。细胞迁移速率在3 M NaCl处理的多层膜上最慢，与组织培养板上的细胞迁移速率相当，但在5 M NaCl处理的多层膜上最高。进一步研究了细胞粘附、增殖、粘附及相关基因的表达，以阐明其内在机制。SMC更喜欢附着，蔓延和增殖的PSS为主的表面上有组织的粘着斑和肌动蛋白纤维，特别是在3 M NaCl处理的多层膜，而保持圆形，并表现出低活力的PDADMAC为主的表面。粘附相关基因，如纤连蛋白，层粘连蛋白和黏着斑激酶，和迁移相关基因，如肌球蛋白IIA和Cdc 42的相对mRNA表达水平进行了比较，以解释不同的细胞行为。这些结果表明，表面化学和盐处理的多层膜的溶胀支配细胞迁移行为。
The cell migration plays a crucial role in a variety of physiological and pathological processes and can be regulated by the cell-substrate interactions. We found previously that the poly (sodium 4-styrenesulphonate) (PSS)/poly(diallyldimethylammonium) chloride (PDADMAC) multilayers post-treated in 1-5 M NaCl solutions result in continuous changes of their physicochemical properties such as thickness, chemical composition, surface charge, swelling ratio and wettability. In this study, the responses of human smooth muscle cells (SMCs) on these salt-treated multilayers, particularly the governing factors of cellular migration that offer principles for designing therapeutics and implants, were disclosed. The cell migration rate was slowest on the 3 M NaCl-treated multilayers, which was comparable with that on tissue culture plates, but it was highest on 5 M NaCl-treated multilayers. To elucidate the intrinsic mechanisms, cell adhesion, proliferation, adhesion and related gene expressions were further investigated. The SMCs preferred to attach, spread and proliferate on the PSS-dominated surfaces with well-organized focal adhesion and actin fibres, especially on the 3 M NaCl-treated multilayers, while were kept round and showed low viability on the PDADMAC-dominated surfaces. The relative mRNA expression levels of adhesion-related genes such as fibronectin, laminin and focal adhesion kinase, and migration-related genes such as myosin IIA and Cdc42 were compared to explain the different cellular behaviours. These results reveal that the surface chemistry and the swelling of the salt-treated multilayers govern the cell migration behaviours.