Radiation dose optimized lateral expansion of the field of view in synchrotron radiation X-ray tomographic microscopy.
Radiation dose optimized lateral expansion of the field of view in synchrotron radiation X-ray tomographic microscopy.
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DOI：
10.1107/s0909049510019618
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发表时间：
2010-09
影响因子：
2.5
通讯作者：
Stampanoni M
中科院分区：
文献类型：
--
作者：
Haberthür D;Hintermüller C;Marone F;Schittny JC;Stampanoni M
增加基于断层摄影的成像方法的横向视场大大增加了采集时间。本文介绍了扫描协议，以获得高分辨率的断层扫描与大的横向视野，大大减少采集时间，从而减少辐射剂量，同时产生高质量的三维断层数据集。微米级分辨率的体积数据可以在几分钟内使用同步辐射断层扫描显微镜获得。通过叠加多个断层图像，可以轻松增加沿样品旋转轴沿着的视场，从而以高分辨率研究细长物体。相反，视场在垂直方向上的扩展是重要的。本文提出了一种采集协议，它增加了垂直于其旋转轴的断层数据集的视野。采集协议可以根据重建质量和扫描时间进行调整。由于扫描时间与给予样品的辐射剂量成比例，因此该方法可用于增加断层扫描显微镜仪器的视场，同时优化辐射敏感样品的辐射剂量并将断层扫描数据集的质量保持在所需水平。这种方法被称为宽场同步辐射断层扫描显微镜，可以将横向视野增加五倍。该方法已成功应用于直径为4.1 mm、体素尺寸为1.48 µm的整个大鼠肺腺泡的三维成像。
Increasing the lateral field of view of tomography-based imaging methods greatly increases the acquisition time. This article presents scanning protocols to obtain high-resolution tomographic scans with large lateral field of view at greatly decreased acquisition time and thus reduced radiation dose while resulting in high-quality three-dimensional tomographic datasets. Volumetric data at micrometer level resolution can be acquired within a few minutes using synchrotron-radiation-based tomographic microscopy. The field of view along the rotation axis of the sample can easily be increased by stacking several tomograms, allowing the investigation of long and thin objects at high resolution. On the contrary, an extension of the field of view in the perpendicular direction is non-trivial. This paper presents an acquisition protocol which increases the field of view of the tomographic dataset perpendicular to its rotation axis. The acquisition protocol can be tuned as a function of the reconstruction quality and scanning time. Since the scanning time is proportional to the radiation dose imparted to the sample, this method can be used to increase the field of view of tomographic microscopy instruments while optimizing the radiation dose for radiation-sensitive samples and keeping the quality of the tomographic dataset on the required level. This approach, dubbed wide-field synchrotron radiation tomographic microscopy, can increase the lateral field of view up to five times. The method has been successfully applied for the three-dimensional imaging of entire rat lung acini with a diameter of 4.1 mm at a voxel size of 1.48 µm.