Light-induced nicking of deoxyribonucleic acid by cobalt(III) bleomycins.
Light-induced nicking of deoxyribonucleic acid by cobalt(III) bleomycins.
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钴 (III) 博莱霉素对脱氧核糖核酸进行光诱导切口。
DOI：
10.1021/bi00268a001
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发表时间：
1982
期刊：
影响因子：
2.9
通讯作者：
Meares,CF
中科院分区：
文献类型：
--
作者：
Chang,CH;Meares,CF
Materials and MethodsMaterials. BLM, supplied as the clinical mixture Blenoxane, was a generous gift of Bristol Laboratories, Syracuse, NY. 0X174 RF DNA was purchased from Bethesda Research Laboratories and contained> 95% form I. Ethidium bromide andagarose (type I) were purchased from Sigma. Other chemicals were reagent grade. Deionized distilled water was used throughout, and labware was acid washed and thoroughly rinsedto avoid heavy metal contamination. Preparation of Cobalt (III) Bleomycins. CoCl2 (100 mM, 55 pL) was added to a solution of Blenoxane (5.5 mM, 1 mL), and the pH was adjusted to neutralitywith dilute NaOH. The yellowish green reaction mixture, after being allowed to stand at room temperature overnight, was applied to a Sephadex C-25 column (NH4+ form, 1 X 50 cm) and eluted with a linear gradient of 0.05-0.5 M ammonium formate, pH 5.7. The major reaction products were separated into four bands that were eluted at 0.14, 0.23, 0.33, and 0.43 M ammonium for-mate, corresponding to A2 green, B2 green, A2 brown, and B2 brown, respectively.[Bleomycin A2 and bleomycin B2 contain different “terminal amine” residues (Umezawa, 1978).] The green complexes were further purified on a Waters C18 y-Bondapak column with 0.1 M ammonium acetate/acetonitrile (85/15 v/v) as the solvent. The green complexes were identical with those previously described by DeRiemer et al.(1979); the brown Co (III) BLM’s will be described elsewhere (Chang
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DOI：
10.1165/rcmb.2015-0205oc
发表时间：
2016-06-01
影响因子：
6.4
作者：
Cui, Huachun;Banerjee, Sami;Liu, Gang
通讯作者：
Liu, Gang
影响因子：
8
作者：
Stoll BJ;Hansen NI;Bell EF;Shankaran S;Laptook AR;Walsh MC;Hale EC;Newman NS;Schibler K;Carlo WA;Kennedy KA;Poindexter BB;Finer NN;Ehrenkranz RA;Duara S;Sánchez PJ;O'Shea TM;Goldberg RN;Van Meurs KP;Faix RG;Phelps DL;Frantz ID 3rd;Watterberg KL;Saha S;Das A;Higgins RD;Eunice Kennedy Shriver National Institute of Child Health and Human Development Neonatal Research Network
通讯作者：
Eunice Kennedy Shriver National Institute of Child Health and Human Development Neonatal Research Network
DOI：
10.1152/ajplung.00356.2014
发表时间：
2015-08
期刊：
American journal of physiology. Lung cellular and molecular physiology
影响因子：
--
作者：
Yang Wang;Chaoqun Huang;N. Chintagari;Dong Xi;Tingting Weng;Lin Liu
通讯作者：
Yang Wang;Chaoqun Huang;N. Chintagari;Dong Xi;Tingting Weng;Lin Liu
影响因子：
4.6
作者：
Bhaskaran, Manoj;Wang, Yang;Liu, Lin
通讯作者：
Liu, Lin
影响因子：
4.6
作者：
Carraro, Gianni;Shrestha, Amit;Bellusci, Saverio
通讯作者：
Bellusci, Saverio