Multilocus sequence analysis of Porphyromonas gingivalis indicates frequent recombination
Multilocus sequence analysis of Porphyromonas gingivalis indicates frequent recombination
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DOI：
10.1099/mic.0.26267-0
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发表时间：
2003-09-01
期刊：
影响因子：
2.8
通讯作者：
Schmidt, H
中科院分区：
文献类型：
--
作者：
Koehler, A;Karch, H;Schmidt, H
本研究应用多位点序列分析技术对19株牙周炎患者牙龈卟啉单胞菌进行了遗传学分析。用PCR方法扩增了10个染色体基因ef-tu、ftsQ、hagB、gpdxJ、pepO、mcmA、dnaK、recA、pga和nah的内部400-600 bp DNA片段，并进行了序列测定。没有两个分离株在所有10个位点上都是相同的。系统发育分析表明，牙龈卟啉单胞菌的种群结构为全混群。裂解分析、同源性比率计算和聚类多态性分析均表明重组在牙龈卟啉单胞菌遗传异质性的形成中起主要作用。标准化关联指数为0.0898，表明所分析的牙龈卟啉单胞菌基因接近连锁平衡。
In this study, the genetic relationship of 19 Porphyromonas gingivalis isolates from patients with periodontitis was investigated by multilocus sequence analysis. Internal 400-600 bp DNA fragments of the 10 chromosomal genes ef-tu, ftsQ, hagB, gpdxJ, pepO, mcmA, dnaK, recA, pga and nah were amplified by PCR and sequenced. No two isolates were identical at all 10 loci. Phylogenetic analyses indicated a panmictic population structure of P. gingivalis. Split decomposition analysis, calculation of homoplasy ratios and analyses of clustered polymorphisms all indicate that recombination plays a major role in creating the genetic heterogeneity of P. gingivalis. A standardized index of association of 0.0898 indicates that the P. gingivalis genes analysed are close to linkage equilibrium.