Detection and localization of the thyroid hormone receptor beta mRNA in the immature olfactory receptor neurons of chum salmon.
Detection and localization of the thyroid hormone receptor beta mRNA in the immature olfactory receptor neurons of chum salmon.
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DOI：
10.1016/j.heliyon.2018.e00744
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发表时间：
2018-08
期刊：
影响因子：
4
通讯作者：
Mochida K
中科院分区：
文献类型：
--
作者：
Kudo H;Eto A;Abe T;Mochida K
甲状腺激素（Thyroid hormone，TH）在调节脊椎动物多种器官和组织的细胞增殖、细胞死亡和能量代谢等多种细胞和代谢过程中起着重要作用。通常认为，溯河产卵的太平洋鲑鱼（Oncorhynchus spp.）在它们向海的迁移过程中，它们从纳塔尔的河流中留下气味，然后在产卵迁移过程中，它们使用嗅觉来区分它们的纳塔尔河流。在向海迁移过程中，血清TH水平和嗅上皮TH的特异性结合值均显著增加。然而，甲状腺激素受体（TR）的表达在嗅觉上皮尚未得到证实，在脊椎动物。我们研究了鲑鱼TR亚型的基因表达。凯塔）。用逆转录聚合酶链反应（RT-PCR）检测嗅上皮TRβ mRNA的表达。核苷酸序列分析表明，RT-PCR产物与其他硬骨鱼TRβ亚型的部分配体结合域具有显著的同源性。用地高辛标记的鲑鱼嗅觉TRβ cRNA探针进行原位杂交，发现鲑鱼嗅觉TRβ mRNA的信号优先出现在未成熟嗅觉受体神经元（ORN）的核周区，为蛋白基因产物9.5（PGP9.5）免疫阳性的ORN。我们的研究结果提供了TRβ基因在嗅上皮中表达的首次检测，并提示TRβ可能参与太平洋鲑鱼ORN的细胞成熟和/或细胞分化。
Thyroid hormone (TH) plays an important role in regulating multiple cellular and metabolic processes, including cell proliferation, cell death, and energy metabolism, in various organs and tissues of vertebrates. It is generally accepted that anadromous Pacific salmon (Oncorhynchus spp.) imprint odorants from their natal stream during their seaward migration, and they then use olfaction to discriminate their natal stream during the spawning migration. Both serum TH levels and the specific binding values of TH in the salmon olfactory epithelium were markedly increased during the seaward migration. However, thyroid hormone receptor (TR) expression in the olfactory epithelium has not been confirmed in vertebrates. We investigated gene expression of TR isoforms in chum salmon (O. keta) by both molecular biological and histochemical techniques. Expression of TRβ mRNA was detected in the olfactory epithelium by reverse transcriptase polymerase chain reaction (RT-PCR). Nucleotide sequencing demonstrated the existence of a remarkable homology between the RT-PCR product and part of the ligand-binding domain of other teleost TRβ isoforms. By in situ hybridization using a digoxygenin-labeled salmon olfactory TRβ cRNA probe, signals for salmon olfactory TRβ mRNA were observed preferentially in the perinuclear regions of immature olfactory receptor neurons (ORNs), as protein gene product 9.5 (PGP9.5)-immunopositive ORNs. Our results provide the first detection of TRβ gene expression in the olfactory epithelium, and suggested the possibility that TRβ may be involved in cell maturation and/or cell differentiation of the ORNs in Pacific salmon.